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Fruit Cup DNA Extraction

Fruit Cup DNA Extraction. Step 1. Into one of the 5 oz cups add 1 teaspoon of shampoo. Fruit Cup DNA Extraction. Step 2. Add 2 pinches of table salt. Fruit Cup DNA Extraction. Step 3. Add 25 ml of distilled water to make a final volume of 30 ml or about 1/3 the volume of the cup.

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Fruit Cup DNA Extraction

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  1. Fruit Cup DNA Extraction

  2. Step 1 • Into one of the 5 oz cups add 1 teaspoon of shampoo. Fruit CupDNA Extraction

  3. Step 2 • Add 2 pinches of table salt. Fruit CupDNA Extraction

  4. Step 3 • Add 25 ml of distilled water to make a final volume of 30 ml or about 1/3 the volume of the cup. • Dissolve the salt and shampoo by stirring slowly to avoid foaming. Fruit CupDNA Extraction

  5. Step 4 • Using the plastic knife, peel and cut 1/2 of a kiwi into small pieces. Fruit CupDNA Extraction

  6. Step 5 • Using the plastic spoon, add the kiwi to the solution from steps 1-3. Fruit CupDNA Extraction

  7. Step 6 • Mash the kiwi against the side of the cup with the back of the spoon for 10 minutes. Fruit CupDNA Extraction

  8. Step 7 • While one member of your group mashes the kiwi, another member will place a #2 cone coffee filter inside the second 5 oz plastic cup. Fruit CupDNA Extraction

  9. Step 7(cont.) • Fold the coffee filter’s edge around the cup so that the filter does not touch the bottom of the cup. Fruit CupDNA Extraction

  10. Step 8 • Filter the mixture by pouring it into the filter and letting the solution drain for several minutes until there is approximately 5 ml (covers the bottom of the cup) of filtrate to test. Fruit CupDNA Extraction

  11. Step 9 • Obtain a test tube of cold alcohol. For best results, the alcohol should be as cold as possible. Fruit CupDNA Extraction

  12. Step 10 • Fill the plastic pipette with kiwi solution to the 1 ml mark. Fruit CupDNA Extraction

  13. Step 11 • Add kiwi solution from the pipette to the cold alcohol. Fruit CupDNA Extraction

  14. Step 12 • Let the solution sit for 2 to 3 minutes without disturbing it. • It is important not to shake the test tube. Fruit CupDNA Extraction

  15. Step 13 • You can watch the white DNA precipitate out into the alcohol layer. • DNA has the appearance of white, stringy mucus. Fruit CupDNA Extraction

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