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The origin dictates the niche Enrichment and characterisation of open chain elongating communities

The origin dictates the niche Enrichment and characterisation of open chain elongating communities Pieter Candry , Shengle Huang , José Maria Carvajal-Arroyo, Korneel Rabaey, Ramon Ganigué – 26/06/19. Caproic acid. Caproic acid. Chemical building block. Transportation fuels.

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The origin dictates the niche Enrichment and characterisation of open chain elongating communities

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  1. The origin dictates the niche Enrichment and characterisation of open chain elongating communities Pieter Candry, Shengle Huang, José Maria Carvajal-Arroyo, Korneel Rabaey, Ramon Ganigué – 26/06/19

  2. Caproic acid

  3. Caproic acid Chemical building block Transportation fuels Antimicrobial in animal feed

  4. Caproic acid

  5. Ethanol Acetic acid Clostridium kluyveri Caproic acid

  6. Energy crops Lignocellulosics Organic wastes Primary fermentation Bio-ethanol Ethanol Acetic acid Clostridium kluyveri Caproic acid

  7. Syngas (H2/CO2/CO) Gas fermentation Ethanol Acetic acid Clostridium kluyveri Caproic acid

  8. pH is a key operational parameter in chain elongation pKa = 4.8 Methanogen inhibition + Methanogen inhibition - Toxicity + Toxicity - - (in-situ) separation - (in-situ) separation + pH 5.5 pH 7

  9. Chain elongation in Clostridium kluyveri DSM555 3231B µmax = approx. 0.13 h-1 pH = 4.8-9.2 µmax = 0.24 ± 0.01 h-1 pH = 6.0 - 7.5 Weimer et al. (2012), Appl. Microbiol. Biotechnol. 94 Candry et al. (2018), S.Rep 8(9724)

  10. Mixed chain elongating communities pH 5.5 pH 7 Hegner et al. (2017), RSC Adv. 7(15362) Kucek et al. (2016), EES 9( 3482)

  11. Mixed chain elongating communities pH 7 pH 5.5 Hegner et al. (2017), RSC Adv. 7(15362) Kucek et al. (2016), EES 9( 3482)

  12. What natural and engineered niches do chain elongators inhabit? What is the impact of pH on the enriched community? What is the impact of the origin on the obtained community?

  13. AD Ossemeersen (Os) Thin stillage pilot (TS) Goat feces (Go) Sheep feces (Sh) UASB Van Steenberghe (VS) UASB Lindemans (Li) Inocula

  14. AD Ossemeersen (Os) Thin stillage pilot (TS) Goat feces (Go) Sheep feces (Sh) UASB Van Steenberghe (VS) UASB Lindemans (Li) Inocula Li 7 1 VS 7 1 Os 7 1 Li 5.5 1 TS 7 1 VS 5.5 1 Go 7 1 Sh 7 1 Sh 5.5 1 TS 5.5 1 Os 5.5 1 Go 5.5 1 Conditions Li 5.5 2 VS 5.5 2 Os 5.5 2 Go 5.5 2 Sh 5.5 2 TS 5.5 2 Li 7 2 VS 7 2 Os 7 2 Go 7 2 Sh 7 2 TS 7 2

  15. Ethanol 15 mL.L-1 (257 mM) Acetate 2.5 g.L-1 (42 mM) BES 25 mM MES 25 mM Temp 34ºC Incubation time First transfer 10 days Subsequent 7 days

  16. AD Ossemeersen (Os) Thin stillage pilot (TS) Goat feces (Go) Sheep feces (Sh) UASB Van Steenberghe (VS) UASB Lindemans (Li) Inocula Li 7 1 VS 7 1 Os 7 1 Li 5.5 1 TS 7 1 VS 5.5 1 Go 7 1 Sh 7 1 Sh 5.5 1 TS 5.5 1 Os 5.5 1 Go 5.5 1 Conditions Li 5.5 2 VS 5.5 2 Os 5.5 2 Go 5.5 2 Sh 5.5 2 TS 5.5 2 Li 7 2 VS 7 2 Os 7 2 Go 7 2 Sh 7 2 TS 7 2

  17. AD Ossemeersen (Os) Thin stillage pilot (TS) Goat feces (Go) Sheep feces (Sh) UASB Van Steenberghe (VS) UASB Lindemans (Li) Inocula Li 7 1 VS 7 1 Os 7 1 Li 5.5 1 VS 5.5 1 Go 7 1 Sh 7 1 TS 5.5 1 Os 5.5 1 Conditions Li 5.5 2 Os 5.5 2 Li 7 2 VS 7 2 Os 7 2 Sh 7 2

  18. Anaerobic digesters Fermentation Gut AD Ossemeersen (Os) Thin stillage pilot (TS) Goat feces (Go) Sheep feces (Sh) UASB Van Steenberghe (VS) UASB Lindemans (Li) Inocula Li 7 1 VS 7 1 Os 7 1 Li 5.5 1 VS 5.5 1 Go 7 1 Sh 7 1 TS 5.5 1 Os 5.5 1 Conditions Li 5.5 2 Os 5.5 2 Li 7 2 VS 7 2 Os 7 2 Sh 7 2

  19. Anaerobic digesters Fermentation Gut AD Ossemeersen (Os) Thin stillage pilot (TS) Goat feces (Go) Sheep feces (Sh) UASB Van Steenberghe (VS) UASB Lindemans (Li) Inocula Li 7 1 VS 7 1 Os 7 1 Li 5.5 1 VS 5.5 1 Go 7 1 Sh 7 1 TS 5.5 1 Os 5.5 1 Conditions Li 5.5 2 Os 5.5 2 Li 7 2 VS 7 2 Os 7 2 Sh 7 2

  20. Anaerobic digesters Fermentation Gut AD Ossemeersen (Os) Thin stillage pilot (TS) Goat feces (Go) Sheep feces (Sh) UASB Van Steenberghe (VS) UASB Lindemans (Li) Inocula Li 7 1 VS 7 1 Os 7 1 Li 5.5 1 VS 5.5 1 Go 7 1 Sh 7 1 TS 5.5 1 Os 5.5 1 Conditions Li 5.5 2 Os 5.5 2 Li 7 2 VS 7 2 Os 7 2 Sh 7 2

  21. Anaerobic digesters Fermentation Gut AD Ossemeersen (Os) Thin stillage pilot (TS) Goat feces (Go) Sheep feces (Sh) UASB Van Steenberghe (VS) UASB Lindemans (Li) Inocula Li 7 1 VS 7 1 Os 7 1 Li 5.5 1 VS 5.5 1 Go 7 1 Sh 7 1 TS 5.5 1 Os 5.5 1 Conditions Li 5.5 2 Os 5.5 2 Li 7 2 VS 7 2 Os 7 2 Sh 7 2

  22. The origin determines pH preferences of chain elongators

  23. pH 7 pH 5.5

  24. pH 7 pH 5.5

  25. pH 7 pH 5.5

  26. pH 7 pH 5.5 6.4±1.6 g caproic acid.L-1 2.3±1.8 g caproic acid.L-1

  27. pH 5.5 pH 7

  28. pH 7 pH 5.5

  29. Growth pH determines product concentration but not production rate

  30. pH 5.5 pH 7 Clostridium kluyveri-OTU present in each enrichment

  31. Heteroscedasticity of communities at neutral and acidic pH due to different levels of enrichment

  32. pH 5.5 pH 7 Clostridium kluyveri-OTU present in each enrichment Communities more enriched at acidic pH

  33. Ethanol chain elongators were present in every environment, but had differential pH preferences pH determines product yield, but does not affect production rates Community structure heavily impacted by enrichment pH

  34. Pieter Candry Pieter.candry@ugent.be +3292645976

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