Innovations in Carbohydrate Enzymology Research

1. CEBZB Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Dúbravská cesta 9, SK-84538 Bratislava, Slovak Republic Department of Carbohydrate Enzymology Head: Jozef Nahálka, PhD Laboratory of Synthetic Biology: Nahálka J., Hrabárová E., Talafová K. Laboratory of microbial degradation of plant cell walls: Biely P., Vršanská M., Puchart V., Šuchová K., Fraňová, L. selected paper Nahalka, J., Hrabarova, E., & Talafova, K. (2015). Protein-RNA and protein-glycanrecognitions in light of aminoacidcodes. Biochimica Et BiophysicaActa - General Subjects, 1850(9), 1942-1952. doi:10.1016/j.bbagen.2015.06.013 Biely, P., Sing, S., & Puchart, V. (2016). Towards enzymatic breakdown of complex plant xylan structures: State of the art. BiotechnologyAdvances, 34(7), 1260-1274. doi:10.1016/j.biotechadv.2016.09.001

2. CEBZB Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology,Dúbravská cesta 9, SK-84538 Bratislava, Slovak Republic Ruegeria pomeroyi PPK2 PPK1 PPK3 Industrial biocatalysis – is limited to “energy-independent enzymes” such as hydrolases, which perform relatively simple chemistry.Living cells in nature –are capable of using much more complex chemistry by cofactor-dependent enzymes, especially by nucleoside triphosphate (NTP) ‘‘burning’’ enzymes. We study polyphosphate kinases (PPKs) for NTP regeneration.

3. CEBZB Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology,Dúbravská cesta 9, SK-84538 Bratislava, Slovak Republic Glycosyltransferases – There is no doubt that the oligosaccharides can be used in the prevention, diagnosis and in treatment of chronic diseases. We study glycosyltransferases required for oligosacharide synthesis.

4. CEBZB Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Microbial Degradation of Plant Cell Walls,Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic Hypothetical Plant Xylan and Enzymes Required for Its Hydrolysis PhA (Fe, p-Coum)  Araf Araf Ac 1 1   3 ( )3 ()3 Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl-4Xyl1- 2 2 2   1 Ac 1 MeGlcA MeGlcA  Lignin alcohol  endo--1,4-xylanase (EC 3.2.1.8) -xylosidase (EC 3.2.1.37) or exo--xylanase Xyl1-4Xyl1- -glucuronidase (EC 3.2.1.131) -L-arabinofuranosidase (EC 3.2.1.55)  feruloyl esterase or p-coumaroyl esterase (EC 3.1.1.73)  acetylxylan esterase (EC 3.1.1.72)  glucuronoyl esterase (EC 3.1.1.-)

5. CEBZB Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Microbial Degradation of Plant Cell Walls,Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic Recent findings of the laboratory • Unique combination of catalytic properties of GH30 xylanase IV from Trichoderma reesei – weak endo-action on polymers and release of reducing-end xylose residue from linear oligosaccharides (2013) • Novel eukarytic GH30 glucuronoxylanase (Trichoderma reesei XYN VI) resembling bacterial counterparts (2014) • Migration of acetyl group along xylopyranoside ring (2015) • Complementarity in regioselectivity of CE1-CE7 acetylxylan esterases and CE16 acetyl esterases (2015-2016) • CE16 enzymes are non-reducing-end exo-deacetylases but som show a weak acetylxylan esterase activity (2015-2016) • Novel chromogenic substrates for detection and quantification of glucuronoyl esterase activity (2016) using a b-glucuronidase-coupled assay