1 / 13

B IOMATERIALS L AB N ETWORK D EGRADATION

Join us on February 6th and 7th for a hands-on lab workshop on biomaterials degradation. Learn about PEG-PLA-MA and MSA/MAA network synthesis, testing methods, and applications in biomaterials science.

mlegette
Download Presentation

B IOMATERIALS L AB N ETWORK D EGRADATION

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. BIOMATERIALS LABNETWORKDEGRADATION Monday, February 6th and Tuesday, February 7th

  2. PRE-LAB • Sign-in • Quiz (Metters. 2000)

  3. Rectangular Approximation "Approximating the Area Under a Curve for AP Calculus | Education.com." Education.com | An Education & Child Development Site for Parents | Parenting & Educational Resource. Web. 06 Feb. 2012. <http://www.education.com/study-help/article/rectangular-approximations/>.

  4. Procedural Notes • Teamwork • Take turns pipetting, massing, ect. but make sure you remember what has been added to the solution • Pipetting • Check the volume, change by twisting dial • When filling, go to first stop • When ejecting precursors, go to second stop • When ejecting into syringe mold, go to first stop (to avoid bubbles)

  5. LINEAR MOLECULES • PEG-PLA-MA • MSA MAA (CH2)4

  6. METHACRYLATE POLYMERIZATION Step-growth Polymerization 1 “Arm” …… PEG PLA MA Unlike last lab, each double bond can hook up with TWO other molecules. (Lin, C.C., Pharmaceutical Research. 26(3): 631-643. 2009)

  7. SECTION 1 PEG-PLA-MA Network Synthesis • Can surmise from Metters.2000 this will be bulk degrading • Monitor change in network over time… • Size (diameter / height) • Weight • Young’s Modulus • Stereo-microscopy Imaging Polyanhydrides. Wikipedia. 2011

  8. PEG NETWORK SYNTHESIS • 10wt% PEG-PLA-MA in PBS • 10% photo-initiator by volume (LAP) • 40μL placed in 1mL syringe (tip cut off) • to create cylindrical hydrogel sample geometry • exposed to UV-lamp for 10min • Remove and place in PBS • 3 PEG samples

  9. SECTION 2 MSA/MAA Network Synthesis • Glassy polymer • Monitor change in network over time… • Size (diameter / height) • Weight • Young’s Modulus • Stereo-microscopy Imaging Polyanhydrides. Wikipedia. 2011 (CH2)4

  10. MSA/MAA NETWORK SYNTHESIS Unlike PEG-PLA-MA, the MSA/MAA is a bulk reaction that is not polymerized in a liquid phase. • ≈0.25g of MSA/MAA • 0.1wt% DMAP photo-initiator added to PA-MA • Fill mold “sandwich” (glass slide / Teflon / glass slide) • exposed to UV-lamp for 10min • Remove and place in PBS • 1 MSA/MAA sample

  11. TESTING • Young’s modulus in Materials Testing Lab – 5N load cell • Mass – after patting networks dry • Size – use calipers for height and diameter. Don’t squish hydrogel • All three will be assembled and posted on blackboard. • Stereo-microscopy Imaging – collect images with digital camera • Each group is responsible for collecting their own images • All performed on D0, D1, D3, D8, D10

  12. Big Picture • Control of mechanical properties & degradation behavior allows tuning to your application • Ex: Control degradation rate  Control drug release rate • Bulk • Typically hydrophilic  Biologically inert • Large number of available chemistries • Surface • Typically hydrophobic  Protein adsorption, cell interaction • Constant turnover at surface  difficult to integrate with tissue • Applications • Prevent postsurgical adhesion formation • In vivo drug and protein delivery • Temporary scaffold for tissue repair • Degradable sutures

  13. SECTION 3 Degradation time-course • Look at how networks degrade over time • 1 team member per data collection day

More Related