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B IOMATERIALS L AB I NTRODUCTION AND S AFETY

B IOMATERIALS L AB I NTRODUCTION AND S AFETY. Wednesday, January 18 th. BME 245/445 – Lab 0. P REFACE. The goal of this course is to provide students with hands on experience in… Materials synthesis Materials characterization Materials modification Cell culture Histological Analysis

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B IOMATERIALS L AB I NTRODUCTION AND S AFETY

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  1. BIOMATERIALS LABINTRODUCTIONAND SAFETY Wednesday, January 18th BME 245/445 – Lab 0

  2. PREFACE The goal of this course is to provide students with hands on experience in… • Materials synthesis • Materials characterization • Materials modification • Cell culture • Histological Analysis • Technical Writing & Data Analysis 01/18/2012

  3. EXPECTATIONS • SAFETY! • arrive prepared for lab • work in TEAMS of 4-5 to complete lab • write INDIVIDUAL lab report • provide constructive feedback 01/18/2012

  4. OVERVIEW • Safety • Lab Outline • Expectations • Reports 01/24/2012

  5. SAFETY • Environmental Health & Safety Training for Biological/Chemical Research Labs • labs are dangerous; so be safe • if you have questions…ASK • DON’T BE STUPID 01/18/2012

  6. CHEMICAL HAZARDS • Flammable / Combustible / Toxic • Respect all chemicals in lab • If you can smell it…it is bad for you! • Use a hood if specified • LABEL EVERYTHING! 01/18/2012

  7. BIOHAZARDS • Biosafety Level 1 – least hazardous • Cell Culture • Media Preparation • LABELS! (krishgen.com) 01/18/2012

  8. PROTECTIVE EQUIPMENT • Goggles – on at ALL times! • Gloves – use them for everything • Closed-toe shoes • Safety Quiz (sentrysafetysupply.com) 01/18/2012

  9. BIOMATERIALS LABS • Biomaterial Mechanics • Bulk vs. Surface Degradation • Cell-Material Interactions • Controlled Delivery and Release • Histological Analysis of Implanted Biomaterials 01/18/2012

  10. 1. BIOMATERIAL MECHANICS • Investigate how hydrogel precursors influence end-stage network mechanics 01/18/2012

  11. 2. DEGRADATION MECHANISMS • Investigate different network chemistries and how these influence degradation (Wikipedia - Anhydride. 2011) 01/18/2012

  12. 3. CELL INTERACTIONS • Investigate how hydrogel incorporated “RGD” peptide influences cell adhesion (U. Michigan ME – Jianping Fu. 2011) 01/24/2011

  13. 4. DELIVERY AND RELEASE • Investigate how hydrogel properties can be modified to control drug delivery Large Mesh Size Small Mesh Size 01/18/2012

  14. 5. HISTOLOGICAL ANALYSIS OF IMPLANTED BIOMATERIALS • Investigate how tissue responds to implanted biomaterials (Heungsoo Shin et al. Biomaterials. 24(19) 2011) 01/18/2012

  15. PRE-LAB • Literature reading relevant to lab • Calculations • Brief quiz at start of lab • Introductory “lecture” 01/18/2012

  16. POST-LAB REPORTS • Present findings in clear/concise fashion • Make connections between lab experiments and pre-lab literature reading • List team members • Error analysis • REFERENCES! • See Write-up Expectations on Blackboard 01/18/2012

  17. For Lab #1 • Register: Mon or Tues only • Complete safety quiz; bring to lab (104) • Read paper on Blackboard • Read lab handout • Do pre-lab calculations

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