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Single Fluorescent Nano-diamond, FND , as Bio-marker. Three dimensional tracking in live cell. 35 eV. V. 800 º C annealing. N. N. N. V. 3-MeV proton beam irradiation. V. Wunshain Fann, IAMS. Fluorescence from the nitrogen-vacancy centers is
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Single Fluorescent Nano-diamond,FND, as Bio-marker Three dimensional tracking in live cell 35 eV V 800 ºC annealing N N N V 3-MeV proton beam irradiation V Wunshain Fann, IAMS • Fluorescence from the nitrogen-vacancy centers is stable and no blinking after surface functionalization 0
Three Dimensional Tracking of a Single FNDVertical Feedback Control z-axis nanopositioning Feedback Electronics CCD 1
Three dimensional tracking of a single 35-nm FND in Live He-La cell for 15 mins 3-d Trajectory Fluorescent Bright Field Total Time: 200 secs Speed up to 20 secs 2 This is the longest 3-d tracking of a nano-particle in live cell reported so far