1 / 20

A long time ago in an Operating Room far, far away….

Biofilm Wars. With apologies to George Lucas. A long time ago in an Operating Room far, far away…. Shocking: Elimination of S. Aureus Biofilm from Stainless Steel by Electrolysis Matthew J. Dietz, MD; Jacob Conjeski MD; Vincent L. Kish AS; Brian R. Hamlin, MD

molimo
Download Presentation

A long time ago in an Operating Room far, far away….

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Biofilm Wars With apologies to George Lucas A long time ago in an Operating Room far, far away….

  2. Shocking: Elimination of S. AureusBiofilm from Stainless Steel by Electrolysis Matthew J. Dietz, MD; Jacob Conjeski MD; Vincent L. Kish AS; Brian R. Hamlin, MD West Virginia University Department of Orthopaedics

  3. Orthopaedic Biofilm • Adherent Bacteria • EPS (extra-cellular polysaccharide) • Polymicrobial TOP SECRET

  4. Spork Implant S. Epi S. Aureus EPS

  5. “race for the surface” Bacterial Colonization vs. Tissue Integration 100 - 1000x Antibiotics to Eliminate Biofilm -- Gristina, A. “Biomaterial-Centered Infection: Microbial Adhesion Versus Tissue Integration. Science. Vol. 237, No. 4822. (Sep. 25, 1987), pp. 1588-1595

  6. “Strategerie”

  7. Debridement with Retention Staged Explant/Exchange Resection Arthroplasty Arthrodesis Amputation

  8. Hypothesis

  9. R I P S. aureus BIOFILM

  10. Biofilm and WVU Orthopaedics Create Biofilm --Goeres et. al. “Statistical assessment of a laboratory method for growing biofilms.” Microbiology. 2005; 151: 757-762.

  11. Where’s the Biofilm?

  12. -- Rabinovitch, C et al. “Removal and Inactivation of Staphylococcus epidermidisBiofilms by Electrolysis.” App and Enviromental Microbiology. Sept 2006: 6364-66.

  13. Anode vs. CathodeReduction CFU’s for S. aureus Control 1 Volts Avg. 52.65% reduction in CFU’s/cm2 Anode (n=5) Avg. 92.59% reduction in CFU’s/cm2 Cathode (n=5)

  14. Biofilm and WVU Orthopaedics

  15. Control 4 V 2 V 1 V 0.5 V

  16. However…

  17. Conclusions Need for adjuntive measures Connection to Cathode for S. aureus CFU reduction independent of voltage 98% of CFU’s via electrolysis

  18. To Do List… • Biofilm Models • Detection of Biofilm • Electrolysis • Various Metals • In-vivo • Vary biofilm makeup • Means of Removal

  19. The War Continues… Thank You

More Related