The Relationship of Helicobacter Pylori Infection to Prostate Cancer and

1. 1 2 3 4 5 6 7 8 The Relationship of Helicobacter Pylori Infection to Prostate Cancer and Benign Prostatic Hyperplasia: Is It Real? Mohammed Al-Marhoon, Allal Ouhtit, Krishna Venkiteswaran, Qassim Al-Busaidi, Josephkunju Mathew, Ibrahim Al-Haddabi, Aisha Al-Abri, Omar Shareef , Shahid Aquil, Khalid Rahman, Intisar Al-Hashmi, Shyam Ganguly Sultan Qaboos University - Oman Aim Methods Results Conclusion • To examine whether Helicobacter Pylori (H. pylori) is detectable in both benign prostatic hyperplasia (BPH) and prostate cancer (PCa). • 100 consecutive patients with prostate diseases were enrolled in the study. • Detection of H. pylori DNA in prostate tissue from patient with BPH and PCa was performed using both Immunohistochemistry (IHC) and Polymerase chain reaction (PCR) • The results were confirmed by DNA sequencing. • Odds ratios and Fisher Exact test were used for the analysis. • 78% of the patients had BPH and 19% had PCa. • PCR combined with sequencing detected H. pylori DNA in the prostate tissue sample from 5 patients (Table 1, Figure 1). • Statistically, BPH and PCa are not significantly associated with the presence of H. pylori DNA in the prostate tissue (OR = 0.94, one tail Chi-square value = 0.660, P > 0.05). • The limitation of this study is the small number of PCa patients. • This study provides, for the first time, a molecular evidence for the presence of H. pylori DNA in the prostatic tissue of patients with BPH and PCa. • This study paves the way for further comprehensive studies to look for the association of H. pylori infection with BPH & PCa. Introduction References • Epidemiological studies have shown significant associations between infective chronic prostatitis and prostatic carcinoma. Many bacteria have been found in the prostate of patients with chronic prostatitis, BPH and PCa.1,2,3 • Al-Marhoon4 proposed a hypothetical model relating H. pylori infection to prostate and bladder diseases. Cohen RJ, Shannon BA, McNeal JE, et al: Propionibacterium acnes associated with inflammation in radical prostatectomy specimens: a possible link to cancer evolution? JURO 2005; 173: 1969–1974. de Martel C and Franceschi S: Infections and cancer: established associations and new hypotheses. Crit. Rev. Oncol. Hematol. 2009; 70: 183–194. Elkahwaji JE, Zhong W, Hopkins WJ, et al: Chronic bacterial infection and inflammation incite reactive hyperplasia in a mouse model of chronic prostatitis. Prostate 2007; 67: 14–21. Al-Marhoon MS: Is there a role for Helicobacter pylori infection in urological diseases? Urol J 2008; 5: 139–143. Table 1 Figure 1 Nested PCR: Lane 1: 50-2000 bp Ladder, Lane 2: Positive sample (110bp), Lane3: Positive sample (110bp), Lane 4: Positive sample (110bp), Lane5: Positive sample (110bp), Lane 6: negative sample, Lane 7: 50-2000 bp Ladder, Lane 8: Positive control (H. pylori genomic DNA ,26695) PCR positive patients for H. Pylori (+: Positive) PCR: polymerase chain reaction LUTS: lower urinary tract symptoms BPH: benign prostatic hyperplasia PSA: prostate specific antigen