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Mold Track `n Trace. Mold’ly going where no man has gone before. iGEM 2011 project proposal – Wageningen UR 2011. A. nidulans. Following a signal (eGFP) through a hypha Diffusion of signal carrier through septa. Project - Hyphae. Project - Hyphae. Initial stimulation. Project - Hyphae.
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Mold Track `n Trace Mold’ly going where no man has gone before iGEM 2011 project proposal – Wageningen UR 2011
A. nidulans Following a signal (eGFP) through a hypha Diffusion of signal carrier through septa
Project - Hyphae Initial stimulation
Project - Hyphae Initial stimulation First signal produced Carrier buildup
Project - Hyphae Initial stimulation First signal produced Carrier buildup Carrier diffuses Process start again in cell N+1
Project - Hyphae Initial stimulation First signal produced Carrier buildup Carrier diffuses Process start again in cell N+1 etc.
Proposed mechanisms xpna eGFP-Svac XlnR* XlnR* XlnR* XlnR* XlnR* XlnR* XlnR* xpna XlnR*
Proposed mechanisms UASgal4 eGFP-Svac GAL4 UASgal4 GAL4
GAL4 regulation 3c 3c 3c 3c 80 80 80 80 80 80 4 4 4 4 4 4 10bp 10bp
Combination 3c 3c 3c XpnA GAL3c 80 80 4 4 XpnA XlnR* eGFP-Svac
S.C.O.T New model organism, A. nidulans Visual signal (GFP) System can be subdivided (modular) Proposed RR have been used before Some are even BioBricked In house knowledge about A. nidulans
S.C.O.T Keeping noise from ruining system Tuning the system Lots of constructs need to be made
S.C.O.T Tuning and noise reduction using the GAL system Opportunity to gain fundamental knowledge about A. nidulans hyphae Opportunity to broaden the scope of synthetic biology BioBricks
S.C.O.T Long period from transformation until measuring of results, 1 Month. Unknown synthetic territory Eukaryote chassis