1 / 18

Knop : Knop’s solution, inorganic salt nutrition

Knop : Knop’s solution, inorganic salt nutrition. 1902 Haberlandt (Austria): Cultivate isolated plant cells in vitro on an artificial medium. Concept of “ Totipotency ”.

moswen
Download Presentation

Knop : Knop’s solution, inorganic salt nutrition

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Knop: • Knop’s solution, inorganic salt nutrition

  2. 1902 Haberlandt (Austria): Cultivate isolated plant cells in vitro on an artificial medium Concept of “ Totipotency ” Plants can be separated into their component parts ( organs, tissues, or cells ), which can be manipulated in vitro and then grown back to complete plants. Father of Plant Tissue Culture 1922 Kotte (student of Haberlandt): Improve medium : sugar, Organic N-compounds

  3. Robbins (USA) : • Root tip culture - Pisum , Zea , and Gossypium • First success of culturing plant tissue in vitro • 1923 Robbins & Maneval: • Maintained maize roots for 20 weeks with the aid of • subcultures • 1926 Went: • Discovery of auxin • 1933Kogl, Haagen-Smit & Erxleben : • Isolation of IAA (indo-3-acetic acid)

  4. Gautheret • 1932Gautheret: • Root tip & root fragment culture • Gautheret: • Cambium culture ( woody plant ) • It can be maintained several months • Gautheret: • IAA promotes root growth • 1939 Gautheret: • Carrot explants develop to • undifferentiated mass (callus) and it •   can be maintained by repeated • subculture

  5. 1932 Root tip culture - Triticum 1934Indefinite culture of isolated roots Tomato - subculture / 7 days 1936 160 passages Importance of vitamin B complex in root culture ( use yeast extract ) 1939Procambial tissue culture  callus ( Nicotiana ) from small leaf and shoot 1942 Tumor culture  calluswithout hormone White

  6. Steward • Steward: • Carrot culture : • Quantitative analysis of culture growth • Discovered embryogenesis in vitro 1955 Steward & Shantz (in Cornell): Carrot root phloem in liquid medium  single cell suspension 1958 Whole plant ＊ Prove the concept of totipotency

  7. Carrot plants from root cells (Stewart, 1964)

  8. 1952Morel & Martin: First virus-free plant through shoot tips culture ( Dahlia ) 1960 Shoot tip culture of Cymbidium ＊ Propagation many plants from one plant

  9. 1954 Muir, Hildebrandt & Riker (in Wisconsin) : Suspension culture by reciprocal shaker( Nicotiana ) Single cell clone by nurse cell culture method

  10. 1955 Miller and Skoog Discovery of kinetin (the first cytokinin) Tobacco culture ---> bud formation 1957 Skoog & Miller : ＊auxin / cytokinin balance in root or shoot formation high kinetin ---> shoot high auxin ---> root

  11. Auxin / Cytokinin balance

  12. 1956 Nickell (Chas, Pfizer & Co. N.Y.): Single cell suspension culture of Phaseolus vulgaris ＊ Idea of producing plant alkaloids from tissue culture 1957 Tulecke : Pollen culture of Ginkgo biloba 1960 Bergmenn :Plating culture Nicotiana tobacum & Phaseolus vulgaris Single cell suspension culture --> plating in 0.6% agar medium, thin layer in petri dish --> cell divided to form colony

  13. Cocking :(UK) • Enzymatic isolation and • culture of protoplast

  14. 1962 Murashige & Skoog : Tobacco tissue culture • Mineral media: • 1. Macro element • 2. Micro element included Fe • 3. Vitamin • Micropropagation • ＊Development of MS medium

  15. Tobacco plants from single cells(Vasil & Hilderbrandt, 1965)

  16. Guha & Maheshwari :(India) • Anther culture ---> haploid plant • ( Datura ) ＊ One pollen grain ---> one plant • Niizeki & Oono :(Japan) • Haploid plant of rice • ＊ Started for plant breeding

  17. Nagata & Takebe :(Japan) • Plant regeneration from • tobacco protoplast • Carlson : • Somatic hybrid plant • from protoplst fusion • Nicotiana glauca + N. langsdorffii • ( 2n = 24 ) ( 2n = 18 ) • Somatic hybrid • (2n = 42 )

  18. Continue ………………… 1974: Zaenen (Ghent University, Belgium) Discovery of Ti plasmid in Agrobacterium tumefaciens 1970-80s: Nester (USA), van Montago (Ghent Univ., Belgium) Ti plasmid analysis 1983: van Montago, Ghent University, Belgium) First transgenic plant. 1985: Monsanto (USA) Leaf disk transformation method (Monsanto)

More Related