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Virology interests for the Maule Lab

Explore factors influencing plant cell communication during potyvirus infections in peas, focusing on recessive resistance genes and host responses. Collaborative opportunities for research and strategies for marker-assisted selection.

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Virology interests for the Maule Lab

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  1. Virology interests for the Maule Lab • Factors regulating plant cell-to-cell communication • Host transcriptional and biochemical responses to • virus infection • Recessive resistance to potyvirus infection -------

  2. Pea seed-borne mosaic virus (PSbMV; Potyvirus)

  3. Natural resistance genes • Early ‘70s - natural resistance discovered in • Ethiopian pea accessions • Recessive resistances to potyviruses clustered • at multiple loci • Linkage group VI: • - PSbMV - P1 (sbm-1) • - PSbMV - P4 (sbm-4) • - PSbMV - L/P2 (sbm-3) • - Clover yellow vein virus (cyv-2) • - White lupin mosaic virus (wlv) • Linkage group II: • - PSbMV L1 (sbm-2) • - Bean yellow mosaic virus (mo) • - Bean common mosaic virus (bcm) • - Pea mosaic virus (pmv)

  4. Summary of research findings • sbm1 and sbm11 are mutant alleles of eIF4E. • The mutations lie in and around the m7G-cap –binding • pocket and interfere with cap-binding and protein • translation in yeast. • eIF4E as a susceptibility factor in pea has the unusual • role of trafficking with the virus from cell to cell and could • assist with virus cell-to-cell movement

  5. Structure of VPg Plochocka et al (1996) PNAS 93:12150

  6. Opportunities for collaboration • Exchange of information prior to publication • Sharing of crystallographic experiences/expertise

  7. Strategies for delivery • Reagents for marker-assisted selection for crops • Analysis of the molecular interaction between • VPg and eIF4E - crystallisation of VPg and complex • Prediction of resistance alleles for any potyvirus • -host interaction, and identification using TILLING

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