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Martina Janoušková, Institute of Botany ASCR, Czech Republic

Dr. Diederik van Tuinen, UMR INRA-1088; CNRS-5184; U. de Bourgogne INRA-CMSE, Plant-Microbe-Environment Dijon France. Martina Janoušková, Institute of Botany ASCR, Czech Republic. Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un écosysystème hostile.

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Martina Janoušková, Institute of Botany ASCR, Czech Republic

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  1. Dr. Diederik van Tuinen, UMR INRA-1088; CNRS-5184; U. de Bourgogne INRA-CMSE, Plant-Microbe-Environment Dijon France Martina Janoušková, Institute of Botany ASCR, Czech Republic Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un écosysystème hostile

  2. Experimental design PLANTS Tripleurospermum inodorum Calamagrostis epigejos Sisymbrium loeselii AM FUNGI G. intraradices BEG140 G. intraradices BEG141 G. claroideum BEG96

  3. PLANTS Tripleurospermum inodorum Calamagrostis epigejos 10 replicates Harvest at 4 and 8 weeks after inoculation AM FUNGI Non-inoculated G. intraradices BEG140 G. claroideum BEG96 G. intraradices BEG140 + G. claroideum BEG96 ANALYSES Fresh and dry weight Level of colonisation by AM fungi (Trypan blue) Alkaline phosphatase activity Intensity of root colonisation by each fungus (PCR) Expression of selected fungal genes ofG. intraradices (qPCR)

  4. Working on degraded experimental sites. Big collection of AM fungi isolated from degraded environments. Developed molecular tools to monitor fungal gene expression in plant roots, and to detect the fungus in the roots. Why collaborate?

  5. Use of the molecular tools to monitor and assess the fungal fitness in field conditions Selection of new markers appropriated for fungal efficiency monitoring Results and future Glomus intraradices genome sequencing project

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