Viral Vector Core

1. Viral Vector Core Cincinnati Children’s Research Foundation help-cores@bmi.cchmc.org ™

2. CORES Billing: User Training How do I use the system? • Log in to CORES Billing • Select your role • Place an order for the Viral Vector Core • Monitor the status of your order

3. CORES Billing: User Training How do I use the system? • Log in to CORES Billing • Select your role • Place an order for the Viral Vector Core: • Monitor the status of your order

4. CORES Billing: User Training Logging in to CORES Billing Log in to CORES Billing using your CCHMC, UC or external* credentials: https://cores-cchmc.mis.vanderbilt.edu • External users: *log-in credentials will be emailed to you when your account is created. • If you need to request an account or have trouble logging in, please contact help-cores@bmi.cchmc.org

5. CORES Billing: User Training CCHMC & External (non-UC) users enter credentials here UC users click here

6. CORES Billing: User Training How do I use the system? • Log in to CORES Billing • Select your role • Place an order for the Viral Vector Core • Monitor the status of your order

7. CORES Billing: User Training Selecting your role Proxy to the PI PI • Lab member: • Technician • Grad student • Postdoc

8. CORES Billing: User Training What role do I choose?

9. CORES Billing: User Training How do I use the system? • Log in to CORES Billing • Select your role • Place an order from the Viral Vector Core • Monitor the status of your order

10. CORES Billing: User Training How to place an order: Viral Vector Core Log in to CORES Billing Enter your vector order directly in the system

11. CORES Billing: User Training Log in to CORES Billing

12. CORES Billing: User Training Quick Links: Order Entry

13. CORES Billing: User Training Select a Budget Number

14. CORES Billing: User Training Select a Budget Number Please ask your PI which budget number you should use for each transaction.

15. CORES Billing: User Training Select a Budget Number If your budget number is not listed, please contact help-cores@bmi.cchmc.org

16. CORES Billing: User Training Select a Budget Number CCHMC Budget Number format: BUS UNIT  FUND  DEPT  (PROJ  BUDG REF)  CORES Example: 31.90000.590000.(123456.00).ABCD

17. CORES Billing: User Training Select a Budget Number External Account Number format: Generic, 10-digit number for all of your CORES purchases Example: 9999999892

18. CORES Billing: User Training Select a Template

19. CORES Billing: User Training Select a Template

20. CORES Billing: User Training Customize your order using the dropdown menus

21. CORES Billing: User Training Customize your order using the dropdown menus

22. CORES Billing: User Training Customize your order using the dropdown menus

23. CORES Billing: User Training Delete line items not needed

24. CORES Billing: User Training Add any additional items

25. CORES Billing: User Training Add any additional items

26. CORES Billing: User Training Customize using the dropdown menus

27. CORES Billing: User Training Customize using the dropdown menus

28. CORES Billing: User Training Follow instructions in the Comments • Please add: • IBC Number • Plasmid Name • DNA Concentration • FedEx Number (if applicable)

29. CORES Billing: User Training Adjust unit quantities

30. CORES Billing: User Training Select (single unit) versus (multi-unit) If quantity is 2 or more, select (multi-unit) instead of (single unit) to obtain multi-unit discount

31. CORES Billing: User Training Attach your Plasmid Map

32. CORES Billing: User Training Submit Order Request

33. CORES Billing: User Training How to place an order: Viral Vector Core • To review: • Log in to CORES Billing • Quick Links: Order Entry • Select a budget number • Select a Template • Customize order using dropdown menus • Add items if necessary • Follow instructions in Comments: add IBC number and DNA concentration • Attach plasmid map • Submit order request

34. CORES Billing: User Training Comparison to previous VVC Request Form

35. CORES Billing: User Training PI and lab contact information:

36. CORES Billing: User Training PI and lab contact information:is automatically loaded PI Lab contact (person logged in to CORES Billing, placing the order)

37. CORES Billing: User Training Previously: enter up to 4 vector requests 1 2 3 4

38. CORES Billing: User Training CORES Billing: 1 vector/order request 1

39. CORES Billing: User Training CORES Billing: 1 vector/order request 1 Add details

40. CORES Billing: User Training CORES Billing: 1 vector/order request 1 Attach plasmid map

41. CORES Billing: User Training CORES Billing: 1 vector/order request 1 Submit the request for Vector #1

42. CORES Billing: User Training Additional Order Details:

43. CORES Billing: User Training Plasmid Details: Comments and Plasmid map • To Comments add: • Name/Identification • DNA Concentration Add Annotated Plasmid Map that indicates plasmid size

44. CORES Billing: User Training CORES Billing: 1 vector/order request 2 Repeat the process for any additional vectors preps.

45. CORES Billing: User Training Vector Details: Category & Description • Use Category & Description dropdown menus to indicate: • Virus type (+ pseudotype) • Titration • Purification

46. CORES Billing: User Training Add Items: Category & Description Click “Add Item” to request additional items or services:

47. CORES Billing: User Training Add Items: Category & Description Click “Add Item” to request additional items or services:

48. CORES Billing: User Training Add Items: Category & Description • Click “Add Item” to request additional items or services: • Vector Concentration

49. CORES Billing: User Training Add Items: Category & Description • Click “Add Item” to request additional items or services: • Vector Concentration • Plasmid Prep

50. CORES Billing: User Training Add Items: Category & Description • Click “Add Item” to request additional items or services: • Vector Concentration • Plasmid Prep • Safety Testing