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Topic : Microscope Aim : How can we measure a cell using a microscope? Do Now: 1. Take out Density HW ditto 2. Complete the top of yesterday’s notes sheet. HW : Castle Learning due tomorrow!. How do images appear?. Upside down & backwards. On the slide.
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Topic: Microscope Aim: How can we measure a cell using a microscope? Do Now: 1. Take out Density HW ditto 2. Complete the top of yesterday’s notes sheet. HW: Castle Learning due tomorrow!
How do images appear? Upside down & backwards On the slide Seen through lens F
What happens when you move the slide? Image moves in opposite direction
What will happen to the number of cells seen in your field of view when switching to high power?
Units used to measure cells • Micrometers/ Microns • µm • 1mm = 1000µm
Paper thickness = about 150 µm
Human hair diameter = 80-100 µm
mm µm • Move decimal three places to the RIGHT • Large small
Example: 3.2 mm = ? 3. 2 3200 µm 0 0
0.7634mm = ? µm 0. 7 6 3 4 763.4 µm
µm mm • Move the decimal three places to the LEFT • Small large
Example: 789 µm = ? mm 7 8 9 . .789 mm
53.6 µm = ? mm 0 5 3. 6 .0536 mm
1. 1mm 3.25mm = • Field of view = 3250 µm
2. 500um 1000/2 = 500 1mm = 1000um
3. 1000um 2000/2 = 1000 2 mm = 2000um
4. 1000/2 = 500 500um 250um 500/2 = 250 1000um
5. 1000um 2000/2 = 1000 500um 1000/2 = 500 2000um
6. 500um 250um 125um 1000um
7. 2500um 1250um 625um 5mm = 5000um
8. 1000um 5000/5 = 1000 5mm = 5000um
9. 1000um 7000/7 = 1000 7000um
10. 400um 2000/5 = 400 2000um
Let’s summarize: • Identify the units used to measure cells. • Which way do you move the decimal when converting from mm to um? • Which way do you move the decimal when converting from um to mm? • Describe the two procedures that must be followed before switching to high power. • Explain what happens to the amount of light in your field of view when switching from low to high power.
Determine the length of each cell in um. Diameter = 1mm Diameter = 2000um
Determine the length of each cell in um. Diameter = 1000um Diameter = 2mm