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blp locus (deSaizieu et. al.,)

TIGR4 R6 Induction. Genomic Subarray. SP0523 spr0460 N N. SP0524(blpT) spr0461 C N. SP0525(blpS) spr0462 C N.

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blp locus (deSaizieu et. al.,)

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  1. TIGR4 R6 Induction Genomic Subarray SP0523 spr0460 N N SP0524(blpT) spr0461 C N SP0525(blpS) spr0462 C N SP0526(blpR) spr0463 C N SP0527(blpH) spr0464 C N SP0528(blpC) spr0465 N N SP0529 spr0466 C N SP0530 spr0468 IE IE2 SP0531(blpI) N N SP0532(blpJ) C N SP0533(blpK) C N SP0534 N N SP0535 N N SP0536(blpL) I4 N SP0537(IS) C N SP0538(IS) I4(IS) N SP0539(blpM) I 2 C SP0540(blpN) N N SP0541(blpO) N N SP0544(blpX) C C SP0545(blpY) spr0472 IE IE2 SP0546(blpZ) spr0473 IE IE2 SP0547 spr0474 IE IE4 SP0549 spr0475 C N References:1) de Saizieu. et. al., J Bacteriol. 2000 Sep;182(17):4696-703. 2) Hakenbeck R. et. al., FEMS Microbiol Lett. 2000 Sep 15;190(2):231-6. Figure SF3. Cross regulation between the com and blp two-component systems. Deletion in R6 R6 blp locus '1 blpT S 463 ip B B A '0 pncP Z induced by CSP ABC-MSP 464 A Y R6 blp locus (deSaizieu et. al.,) '1 blpT S 463 ip B B A '0 pncP Z induced by BIP-R6 ABC-MSP 464 A Y blp locus (deSaizieu et. al.,) induced by BIP blpT S R H C B A3 A2 I J K L IS M N O X Y Z A1 current annotation for TIGR4 blp locus 523 '4 '5 '6 527 '8 529 530 '1 '2 '3 '6 '7 '8 '9 '0 '1 '4 '5 '6 547 Red arrows = default color for the genes; green arrows = genes induced by CSP or BIP respectively;solid black arrow = invariant expression; hatched black arrow = insertion sequence;blue bar = region present in TIGR4 but absent in R6. Numbers in R6 strain denote spr nos. and in TIGR4 strain denotes SP nos. TIGR4: AE007364; AE007365; R6:AE007317. As suggested before by Hakenbeck (2) and by deSaizieu (1) there is an allelic variation in the blp locus between TIGR4 and R6 strains. Genes annotated as blpI, J, K, L, M, N, O and X, along with some intermediate genes are present in TIGR4 but not in R6. Hakenbeck represented this region as two separate loci, spi and the pnc resp. deSaizieu reported induction of the entire blp cluster of TIGR4 strain in response to its BlpC peptide. Consistent with Hakenbeck's report he also showed that the peptides were strain specific i.e., BlpC of TIGR4 does not induce blp genes in R6 and vice versa. For R6 he reported increased expression of blpA, B, K, U, T and blpZ genes in response to the R6 BlpC peptide. In the present study, we observed induction of some of the blp genes in response to CSP. Genes that increased their expression in response to CSP include SP0530, blpY, Z and SP0547. Some of the genes were invariant (shown in black arrows). It is interesting that these genes are induced early. Since some genes are induced both during competence and by BIP, it seems that there might be some cross talk between these loci.

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