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AquaSentinel: An Advanced Real-Time Biosensor System for Source Water Protection

AquaSentinel: An Advanced Real-Time Biosensor System for Source Water Protection. Elias Greenbaum and Miguel Rodriguez Jr. Chemical Sciences Division Oak Ridge National Laboratory Oak Ridge, Tennessee. 1. Key Points.

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AquaSentinel: An Advanced Real-Time Biosensor System for Source Water Protection

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  1. AquaSentinel: An Advanced Real-Time Biosensor System for Source Water Protection Elias Greenbaum and Miguel Rodriguez Jr. Chemical Sciences Division Oak Ridge National Laboratory Oak Ridge, Tennessee 1

  2. Key Points • --Photosynthetic fluorescence induction is a real-time sentinel assay for measuring algal physiology. • --Toxins that are harmful to humans alter fluorescence induction. • --The specific alteration of fluorescence induction may correlate with the chemical nature of the toxin. 2

  3. Real-World Conditions AquaSentinel Monitoring Station Drinking Water Supply for Oak Ridge, TN CLINCH RIVER/MELTON HILL LAKE, OAK RIDGE, TN

  4. Clinch River Monitoring Station - ORNL

  5. KCN Data Analysis 10 ppm Transformation Data Set for KCN

  6. Transformation Data Set for KCN

  7. Methyl Parathion Data Analysis 4 ppm Transformation Data Set for Methyl Parathion

  8. Transformation Data Set for Methyl Parathion

  9. DCMU Data Analysis – 1 ppm Transformation Data Set for DCMU

  10. Transformation Data Set for DCMU

  11. Paraquat Data Analysis – 0.1 ppm Transformation Data Set for Paraquat

  12. Transformation Data Set for Paraquat

  13. Atrazine Data Analysis – 0.3 ppm Transformation Data Set for Atrazine

  14. Transformation Data Set for Atrazine

  15. Atrazine KCN DCMU Methyl Parathion Paraquat Summary of Toxin Data + Differential Offset Analysis

  16. KCN DCMU Atrazine Paraquat Methyl Parathion Summary of Toxin-Specific Transformation Data Sets

  17. Conclusions • --Photosynthetic fluorescence induction is a real-time sentinel assay for measuring algal physiology. • --Toxins that are harmful to humans alter fluorescence induction. • --The specific alteration of fluorescence induction may correlate with the chemical nature of the toxin. 21

  18. WaterSentryTM + ORNL SensorNet Slide by ORNL IT Manager David E. Hill

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