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CCE Lipidomics studies (2011)

CCE Lipidomics studies (2011). Zhenwen Zhao and Yan Xu 05-27-2011. Brief introduction of WCCE.

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CCE Lipidomics studies (2011)

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  1. CCE Lipidomics studies (2011) Zhenwen Zhao and Yan Xu 05-27-2011

  2. Brief introduction of WCCE The Warfighter Cancer Care Engineering (WCCE) project is a highly innovative, interdisciplinary, multi-institutional, large-scale endeavor that holds promise for revolutionizing the current paradigms of cancer prevention, detection, treatment and care delivery. The project centers on applying system engineering principles to the cancer problem with an initial focus on colon cancer. WCCE has three main goals: 1) to identify molecular signatures in blood that are predictive for colon cancer risk, progression and treatment response, 2) efficiency of care delivery; and 3) the prevention of colon cancer. We focuses on the utilization of molecular data (lipidomic information) derived from cancer and normal blood to identify predictive signatures for the development of cancer and to identify which patients will respond to which treatments. Identification of these molecular signatures is based on HPLC-ESI-MS/MS technology and statistical modeling approaches.

  3. Specimen Collection and Disease Characteristics Cancer Disease Characteristics

  4. Blood Processing and Lipid Extraction Blood samples were collected in EDTA-containing tubes and centrifuged at 1,750g for 15 min at room temperature. Plasma samples were aliquoted into siliconized eppendorf tubes and frozen at -80oC until use. All extraction procedures were performed by Methanol method.

  5. Several lipids were different among the three groups

  6. The major issues/obstacles • In our previous publication, (Zhao, Z., Y. Xiao, P. Elson, H. Tan, S. J. Plummer, M. Berk, P. P. Aung, I. C. Lavery, J. P. Achkar, L. Li, G. Casey, and Y. Xu. 2007. Plasma lysophosphatidylcholine levels: potential biomarkers for colorectal cancer, J. Clin. Oncol. 25: 2696-2701), we have found that LPC levels were significantly different between healthy and CRC cases. However, we did not see the same trend in CCE samples.

  7. Compare the data of total LPC from 3 groups Note: The same procedures were used for samples form CCE and Dr. Cho; however, different method for extraction of lipids and different mass spectrometer were used when analyzing CCF samples.

  8. Validate the LPC data

  9. The correlation of total LPC data between CCF and IUPUI Linear Regression for Data1_B: Y = A + B * X Parameter Value Error ------------------------------------------------------------ A 25.03982 19.99973 B 0.88899 0.05444 ------------------------------------------------------------ R SD N P ------------------------------------------------------------ 0.97648 22.82214 15 <0.0001 ------------------------------------------------------------

  10. Plasma (rat) LPC levels increase 30% after incubation at 37 degree for 1.5 hr. EDTA plasma LPA levels were not signifi cantly changed after 24 h of incubation at 37°C [1]. In contrast, neither heparin nor calcium-trapping anticoagulants (including EDTA and acid citrate dextrose tube) block production of LPC [1,2]. In samples stored 1 day at room temperature, the LPC concentration increased 54% [2], and at 37°C incubation, LPC increased nearly 4-fold [1]. 1. Murph , M. , T. Tanaka , J. Pang , E. Felix , S. Liu , R. Trost , A. K. Godwin , R. Newman , and G. Mills . 2007 . Liquid chromatography mass spectrometry for quantifying plasma lysophospholipids: potential biomarkers for cancer for diagnosis . Methods in Enzymology . 433 : 1 – 25 . 2 . Liebisch , G. , W. Drobnik , B. Lieser , and G. Schmitz . 2002 . Highthroughput quantifi cation of lysophosphatidylcholine by electrospray ionization tandem mass spectrometry . Clin. Chem. 48 : 2217 – 2224 .

  11. Nonfasting plasma (human) LPC levels increase 70%

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