STRUKTUR DAN FUNGSI KROMOSOM

1. STRUKTUR DAN FUNGSI KROMOSOM Niken Satuti Nur Handayani Lab. Genetika Fakultas Biologi UGM

2. Genetics: • From Genes to Genomes • Second Edition • Hartwell ● Hood ● Goldberg ● Reynolds ● Silver ● Veres

3. The bacterial genome is composed of one circular chromosome • 4-5 Mb long • Condenses by supercoiling and looping into a densely packed nucleoid body • Chromosomes replicate inside cell and cell divides by binary fission Fig. 14.4 b

4. Genetics: From Genes to Genomes • Second Edition • Hartwell ● Hood ● Goldberg ● Reynolds ● Silver ● Veres

5. The Eukaryotic Chromosome An Organelle for Packaging and managing DNA

6. Each chromosome packages a single long molecule of DNA • Chemical and Physical analysis • Studies examine stretching and recoiling find longer pieces recoil more slowly than shorter pieces • Pulse field electrophoresis • Separates large pieces of DNA – number and sizes correspond to number and sizes expected if each chromosome contains a single piece of DNA

7. Protein components of DNA • Histone proteins abound the chromatin of all eukaryotic cells • Histones – small proteins with basic, positively charged amino acids lysine and arginine • Bind to and neutralize negatively charged DNA • Make up half of all chromatin protein by weight • Five types: H1, H2A, H2B, H3, and H4 • Core histones make up nucleosome: H2A, H2B, H3, and H4 • DNA and histone synthesis regulation correlate timing so both are synthesized together • High level of similarity of histones among diverse organisms

8. Protein components of DNA • Nonhistone proteins are a heterogeneous group • Half of proteins in chromatin are nonhistone • Large variety of nonhistone proteins – 200 – 2,000,000 in diploid genomes • Large variety of functions • Scaffold – backbone of chromosome • DNA replications – e.g., DNA polymerases • Chromosome segregation – e.g., motor proteins of kinetichores • Transcriptional regulation – largest group regulates transcription during gene expression • Occur in different amounts in different tissues because of variety of function

9. The nucleosome: the fundamental unit of chromosomal packaging arises from DNAs association with histones • Chromatin fibers with beads having diameter of about 100 A and strings having diameter of 20 A

10. The nucleosome:the fundamental unit of chromosomal packaging arises from DNAs association with histones • Bead is a nucleosome with about 160 bp of DNA wrapped twice around a core of 8 histones • 40 bp of DNA link together nucleosomes

11. The nucleosome:the fundamental unit of chromosomal packaging arises from DNAs association with histones • X-ray diffraction analysis • DNA does not coil smoothly • Base sequences dictate preferred nucleosome positions along DNA • Spacing and structure affect genetic function

12. The nucleosome:the fundamental unit of chromosomal packaging arises from DNAs association with histones • Spacing of nucleosomes affects gene expression • Regions between nucleosomes available for interactions with proteins involved in expression, regulation, and further compaction • Determines how and whether certain proteins interact with specific sequences • Packaging into nucleosomes condenses DNA sevenfold • 2 meters of DNA shortens to less than 0.25 meters

13. Models of higher level compaction seek to explain extreme compaction of chromosomes at mitosis • Formation of 300 A fiber through supercoiling

14. Models of higher level compaction seek to explain extreme compaction of chromosomes at mitosis • Radial loop-scaffold model for higher levels of compaction • Each loop contains 60-100 kb of DNA tethered by nonhistone scaffold proteins

15. Radial loop-scaffold model continued

16. Experimental support for radial loop-scaffold model • Electron micrograph shows long DNA loops emanating from the protein scaffold

17. A closer look at karyotypes:fully compacted metaphase chromosomes have unique, reproducible banding patterns • Banding patterns are highly reproducible • Not known what they represent

18. A closer look at karyotypes: fully compacted metaphase chromosomes have unique, reproducible banding patterns. • Banding patterns help locate genes

19. A closer look at karyotypes: fully compacted metaphase chromosomes have unique, reproducible banding patterns • Banding patterns can be used to analyze chromosomal differences between species • Can also be used to reveal cause of genetic disease • e.g., Downs syndrome – 3 copies of chromosome 21

20. Centromere structure and function Fig. 12.11 a

21. Kromosom Eukaryotik dan Prokaryotik AUTOSOM: kromosom yang tidak ada hubungannya dengan penentuan jenis kelamin KROMOSOM KELAMIN (sex chromosome) : kromosom yang ada hubungannya dengan penentuan jenis kelamin

22. Chromosome structure HISTONES are proteins with a high proportion of Positively charged amino acids (lysine and arginin), Which enable them to bind firmly to the negatively Charged DNA doble helix. There are 5 types of histone molecules: H1, H2A, H2B, H3, and H4. Except for H1, they occur in exactly equal numbers. In less condensed chromatin fibers, it becomes apparent that DNA between nucleosomes is bound to H1 histones. DNA in metaphase chromosomes must be shortened 10,000-fold by very efficient packing. This occurs in discrete units, the NUCLEOSOMES, Consisting of DNA and histones.

24. Chromosome • Each chromosome has a constriction point called the centromere, which divides the chromosome into two sections, or arms. • The short arm of the chromosome is labeled the p arm. The long arm of the chromosome is labeled the q arm.

25. Characteristic shapes of chromosomes Short arm Long arm The location of the centromere on each chromosome gives the chromosome its characteristic shape, and can be used to help describe the location of specific genes.

26. BENTUK KROMOSOM 1 2 3 4 Ada empat bentuk kromosom berdasarkan letak sentromer: TELOSENTRIS : sentromer terletak di terminal (bagian ujung kromosom) AKROSENTRIS/SUB TELOSENTRIS : sentromerterletak di sub terminal (mendekati ujung kromosom) SUB METASENTRIS : sentromer terletak di sub median (mendekati bagian tengah) METASENTRIS: sentromer terletak di median (bagian tengah)

27. Nilai Indeks Sentromer Bentuk kromosom Nilai Indeks Sentromer (NIS) p x 100 p + q p = panjang lengan pendek kromosom q = panjang lengan panjang kromosom p+q = panjang absolut kromosom

28. Nilai Indeks Sentromer

29. Karyotype • A display of the paired homologues chromosomes from a cell • Allows determination of: sex of an individual, abnormal chromosome number, other chromosome abnormalities, etc.

36. Banding pattern during metaphase Banding pattern during prophase In humans, each cell normally contains 23 pairs of chromosomes, for a total of 46. The 22 autosomes are numbered by size. The other two chromosomes, X and Y, are the sex chromosomes. How many chromosomes do people have?