1 / 21

La phylogénie : une aide à la physiologie Philippe Monget INRA

La phylogénie : une aide à la physiologie Philippe Monget INRA Physiologie de la Reproduction et des Comportements. Gène d’hyperprolificité BMP-15. Homozygous ewes BMP15 -/- Sterile. Wild type ewes +/- Ovulation rate = 1. Heterozygous ewes BMP15 +/- Ovulation rate = 3. BMP15.

nancy
Download Presentation

La phylogénie : une aide à la physiologie Philippe Monget INRA

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. La phylogénie : une aide à la physiologie Philippe Monget INRA Physiologie de la Reproduction et des Comportements

  2. Gène d’hyperprolificité BMP-15

  3. Homozygous ewes BMP15 -/- Sterile Wild type ewes +/- Ovulation rate = 1 Heterozygous ewes BMP15 +/- Ovulation rate = 3 BMP15 BMP15 BMP15 Complete loss of function Partial loss of function (50%) Ovarian Follicular population in hyperprolific Inverdale, Hanna, Cambridge and Lacaune ewes Inverdale : V31D Hanna : Q23STOP Cambridge : S77F and Q239STOP Lacaune : C53Y

  4. Invalidation of oocyte-expressed BMP-15 in the mouse BMP-15 +/+ -/- +/- …different from BMP-15 -/- ewes

  5. A qualitative or quantitative role of BMP-15 in the regulation of ovulation rate in vertebrates ? Ovulation rate 1-2 6-10 100-5000 15-25 ? ? BMP-15 BMP-15 (defect of processing?) BMP-15 -/-: fertile BMP-15 -/-: sterile

  6. BMP5 BMP6 BMP2 BMPs ubiquistes BMP4 GDF7 BMP15 BMPs ovocytaires GDF9 BMP15 de zebrafish BMP

  7. La fécondation

  8. Fécondation in vitro : différence porc/cheval Pas de pénétration Polyspermie --> Etude comparée de la fécondation

  9. Fixation des spermatozoïdes sur la zone pellucide de l’ovocyte et réaction acrosomique Protéines de la zone pellucide ?

  10. Clarification de la nomenclature des gènes ZP chez les mammifères

  11. ZP1 ZPA ZP2 ZP2 ZPA ZPB ZP3 ZP3 ZPB ZP3a ZPC ZP3B ZPC ZP3b ZP4 ZP4 ZP1 ZP2 ZPA ZP1 ZPA ZP3 ZPB ZP2 ZP3A ZPC ZPC ZP3 ZP4 Clarification de la nomenclature des gènes ZP chez les mammifères

  12. ZP3 Trichosurus vulpecula AAC28736 ZPC/ZP3 Sus scrofa NM_213893 97,90,99 ZPC/ZP3 Bos taurus NM_173974 ZPC/ZP3 Vulpes vulpesAY598032 100,100,100 ZPC/ZP3 Canis familiaris NM_001003224 69,57,90 41,61,98 ZPC Mustela ermineaAY648050 93,83,99 100,100,100 ZPC Mustela putorius furoAY702973 100,*,* 86,*,* ZPC/ZP3 Felis catus NM_001009330 ZPC/ZP3 Callithrix sp. S71825 ZPC Macaca fascicularis AY222644 95,*,* 99,97,100 ZPC/ZP3 Macaca radiata X82639 99,*,* 49,*,* ZPC/ZP3 Homo sapiens NM_007155 ZPC/ZP3 Oryctolagus cuniculus U05782 ZP3 Mesocricetus auratusA60503 ZP3 Microtus brandtiAF304487 99,92,99 ZP3 Lagurus lagurusAF515621 ZPC Notomys alexisAY078054 99,99,99 ZPC Pseudomys australisAY078055 100,99,96 80,65,94 ZP3 Rattus norvegicus NM_053762 99,95,97 100,100, 97 ZP3 Rattus rattus diardiiAY338396 53,*,70 ZPC/ZP3 Mus musculus NM_011776 99,98,94 ZP3 Mus sp.AAB24273 ZPC/ZP3

  13. DMBT1 Canis familiaris ZPD/ ZP1 ZPB/ ZP4 ZPA/ZP2 DMBT1 Sus scrofaNM_001048188 Similar to DMBT1 Bos taurus DMBT1 Mus musculusAB005909 Similar to DMBT1 Homo sapiensXM_940472 Similar to Cuzd1 Canis familiaris XM_544054 Transmembrane protein UO-44D Homo sapiens AAP15458 ERG1 Rattus norvegicus AF167170 Cuzd1 Rattus norvegicus BC090345 Cuzd1 Mus musculusNM_008411 ZP1 Mus musculus NM_009580 ZP1 Rattus norvegicus NM_053509 ZP1 Homo sapiens NM_207341 ZP1 Pan troglodytes XM_522022 ZPB/ZP4 Homo sapiens NM_021186 ZPB/ZP4 Pan troglodytes XM_525105 ZPB/ZP4 Macaca radiata Y10381 ZPB Macaca fascicularis AY222647 ZPB Papio cynocephalus AY222646 ZPB/ZP4 Macaca radiata Y10381 ZPB/ZP4 Callithrix jacchus Y10822 ZPB/ZP4 Bos taurus NM_173975 ZPB/ZP4 Sus scrofa NM_214045 ZPB/ZP4 Oryctolagus cuniculus M58160 ZPB Canis familiaris AY573930 ZPB Mustela ermineaAY779766 ZPB/ZP4 Felis catus NM_001009260 ZPB/ZP4 Rattus norvegicus NM_172330 ZPB Trichosurus vulpeculaAF263013 ZPA Trichosurus vulpeculaAF263014 ZPA Mesocricetus auratusAY876920 ZPA/ZP2 Rattus norvegicus NM_031150 ZPA/ZP2 Mus musculus NM_011775 ZPA/ZP2 Oryctolagus cuniculus L12167 ZPA/ZP2 Felis catus NM_001009875 ZPA/ZP2 Canis familiaris NM_001003304 ZPA/ZP2 Vulpes vulpesAY598031 ZPA/ZP2 Bos taurus NM_173973 ZPA/ZP2 Sus scrofa NM_213848 ZP2 Callithrix jacchus Y10767 ZPA/ZP2 Macaca radiata Y10690 ZPA/ZP2 Macaca fascicularis AY222645 ZPA/ZP2 Pan troglodytes XM_510869 ZPA/ZP2 Homo sapiens NM_003460

  14. ZPA/ZP2 ZPA/ZP2 ZPA/ZP2 ZPB/ZP4 ZPB/ZP4 ZPC/ZP3 ZPC/ZP3 ZPC/ZP3 ZPD/ZP1 ZPD/ZP1 Clarification de la nomenclature des gènes ZP chez les mammifères Chien Bovin Porc Chat Lapin Homme Chimpanzé Rat Souris

  15. Pseudogène ZP4 de souris

  16. DMBT1 Canis familiaris ZPD/ ZP1 ZPB/ ZP4 ZPA/ZP2 DMBT1 Sus scrofaNM_001048188 Similar to DMBT1 Bos taurus DMBT1 Mus musculusAB005909 CUZ/ ZP Similar to DMBT1 Homo sapiensXM_940472 Exprimée dans l’oviducte et l’endomètre ! Similar to Cuzd1 Canis familiaris XM_544054 Transmembrane protein UO-44D Homo sapiens AAP15458 ERG1 Rattus norvegicus AF167170 Cuzd1 Rattus norvegicus BC090345 Cuzd1 Mus musculusNM_008411 ZP1 Mus musculus NM_009580 ZP1 Rattus norvegicus NM_053509 ZP1 Homo sapiens NM_207341 ZP1 Pan troglodytes XM_522022 ZPB/ZP4 Homo sapiens NM_021186 ZPB/ZP4 Pan troglodytes XM_525105 ZPB/ZP4 Macaca radiata Y10381 ZPB Macaca fascicularis AY222647 ZPB Papio cynocephalus AY222646 ZPB/ZP4 Macaca radiata Y10381 ZPB/ZP4 Callithrix jacchus Y10822 ZPB/ZP4 Bos taurus NM_173975 ZPB/ZP4 Sus scrofa NM_214045 ZPB/ZP4 Oryctolagus cuniculus M58160 ZPB Canis familiaris AY573930 ZPB Mustela ermineaAY779766 ZPB/ZP4 Felis catus NM_001009260 ZPB/ZP4 Rattus norvegicus NM_172330 ZPB Trichosurus vulpeculaAF263013 ZPA Trichosurus vulpeculaAF263014 ZPA Mesocricetus auratusAY876920 ZPA/ZP2 Rattus norvegicus NM_031150 ZPA/ZP2 Mus musculus NM_011775 ZPA/ZP2 Oryctolagus cuniculus L12167 ZPA/ZP2 Felis catus NM_001009875 ZPA/ZP2 Canis familiaris NM_001003304 ZPA/ZP2 Vulpes vulpesAY598031 ZPA/ZP2 Bos taurus NM_173973 ZPA/ZP2 Sus scrofa NM_213848 ZP2 Callithrix jacchus Y10767 ZPA/ZP2 Macaca radiata Y10690 ZPA/ZP2 Macaca fascicularis AY222645 ZPA/ZP2 Pan troglodytes XM_510869 ZPA/ZP2 Homo sapiens NM_003460

  17. Clarification de la nomenclature des gènes ZP chez les mammifères: Une nouvelle zp qui vient de l’oviducte ??? ZPA/ZP2 CUZ/ZP ZPC/ZP3 ZPD/ZP1 Cellules épithéliales tubaires

  18. Une famille de gènes en pleine expansion : Les oogénésines

  19. Oogenesin-1 ?? Oogenesin-2 Oogenesin-4 Mm.25370 Mm.148643 Oogenesin-3 50 kb Pramel 1 Mm.31283 Mm.28722 Mm.208554 Mm.26222 Tissue of expression Oocyte Oocyte Oocyte Oocyte Oocyte Oocyte Spermatogonia Methodology RT-PCR In situ hybridization In silico + + + + + + + + + + + + + + +

  20. O T M Lu Sp Br Li K Sk H St G - Oogenesin-2 A Oogenesin-3 Oogenesin-4 Actin O T M Lu Sp Br Li K Sk H B Oogenesin-2 Oogenesin-3 Oogenesin-4 O T C Oogenesin-4 RT-PCR

  21. Perspectives 2007 Coupe du monde de rugby en France

More Related