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The evaluation of Pre-Cut corneas prepared in The International Eye Bank of Prague

The evaluation of Pre-Cut corneas prepared in The International Eye Bank of Prague. Magdaléna Netuková, Yveta Urbanová, Josef Šach, Pavel Kuchynka Ocular Tissue Bank OTB01/The International Eye Bank of Prague, Czech Republic

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The evaluation of Pre-Cut corneas prepared in The International Eye Bank of Prague

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  1. The evaluation of Pre-Cut corneas prepared in The International Eye Bank of Prague Magdaléna Netuková, Yveta Urbanová, Josef Šach, Pavel Kuchynka Ocular Tissue Bank OTB01/The International Eye Bank of Prague, Czech Republic Dpt. of Ophthalmology Vinohrady Teaching Hospital Prague, Czech Republic All authors have No finacial interest

  2. Aim of the study • To evaluate quality of Pre-Cut corneas for DSAEK prepared in the Interantional Eye Bank of Prague • To analyse possible complications during preparation of the Pre-Cut corneas. • To analyse possible complications after preparation of Pre-Cut corneas

  3. Methods - preparation • Pre-Cut corneas were prepared from 30 corneas – not suitable for surgery • All Pre-Cut corneas were preapred in „Clean rooms“ by certified staff members • All Pre-Cut corneas were prepared using standardized method for DSAEK – with Moria microkeratome Moria microkeratome Ljfpqejqoj tpoj Jkpavjpjp ojp

  4. Epithelial abrasion Mounting Pachymetry No major technical problems during preparation Cut Free flap evaluatin Free flap reposition Dismounting

  5. Methods - Evaluation • All Pre-Cut corneas were evaluated using • Slit lamp • Specular microscopy • Selected Pre-Cut corneas were screend for • Microbial comtamination Ljfpqejqoj tpoj Jkpavjpjp ojp

  6. Results - Slit lamp evaluation • All 30 Pre-Cut corneas were evaluated using slit lamp • In 28 Pre-Cut corneas we found good position of the corneal free flap • In 2 cases we foud dislocation of the free flap into the medium • No obvious stromal or endotehelial defects or opacity were detected Incorrect position of the free flap Ljfpqejqoj tpoj Jkpavjpjp ojp Correct position of the free flap

  7. Results Specular microscopy evaluation • The average endothelial cell censity prior PreCut was 2709±483(SD) cells/mm2, • The average endothelial cell censity after PreCut was 2623±407(SD) cells/mm2 • We found no significant endothelial cell loss after preparation. Corneal endothelium after Pre-Cut

  8. Endothelial Cell Density Ljfpqejqoj tpoj Jkpavjpjp ojp

  9. Resuts - Microbiology • 5 randomly selected samples of medium and corneal swaps were microbiologically tested for microbial and fungal contamination – all results were negative • no medium showed signs of bacterial contamination 1 week after preparation of the Pre-Cuts.

  10. Summary • Preparation • In our serie we experienced no major technical problems • The procedure is straight forward with relatively short learning curve • Evaluation • In our serie evaluation of prepared tissue showed high quality and high safety

  11. Conclusion • We believethatour eye bank preparesPre-Cutcorneasforlamelarkeratoplastyof veryhigh quality and safety • We also believe that in the near future Pre-Cut corneas will represent majority of tissues provided by our eye bank

  12. Thankyou International Eye Bank of Prague Srobarova 50 100 34 Prague 10 Czech Republic e-mail:eyebank@fnkv.cz Tel: +420 26716 3678, +420 26716 2267

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