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Asy

A B. Asy. HU release. G 0 /G 1 : 9.7%. miR -NC miR-188. ***. **. **. G 0 /G 1 : 15.6%. C. D. *. **. *. miR -NC miR-188.

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Asy

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  1. A B Asy HU release G0/G1: 9.7% miR-NC miR-188 *** ** ** G0/G1: 15.6% C D * ** * miR-NC miR-188 Supplemental Fig. 3: Stable expression of miR-188 inhibits G1/S transition and Rb phosphorylation. (A) Flow Cytometry analysis of CNE cells stably expressing miR-NC or miR-188 released from hydroxyureafor 6 h. (B) Relative levels of Rb phosphorylation were quantified by densitometricanalysis. Total Rb was used as internal control. Student t test, ** p<0.01, ***p<0.001. (C) Immunoblotanalysis of phosphor-Rb S811, phosphor-Rb S780, total Rb and GAPDH in CNE cells stably expressing miR-NC or miR-188. (D) Relative levels of Rb phosphorylation in (C) were quantified by densitometric analysis , total Rb was used as internal control. Student t test, *p<0.05, ** p<0.01. C1 C2 C1 C2 P-Rb Ser811 P-Rb Ser780 Fig. S3 Total Rb GAPDH

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