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Automatic functional transcriptomic annotation Bioinformatic school CIBA. Manuel Ruiz (CIRAD) Alexis Dereeper (IRD) Marilyne Summo (CIRAD). Transcriptomic data. Genomic expression changes. Signal. expressed proteins Transcriptomic analysis => functions adaptation.
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Automatic functional transcriptomic annotationBioinformatic school CIBA Manuel Ruiz (CIRAD) Alexis Dereeper (IRD) Marilyne Summo (CIRAD)
Transcriptomic data Genomic expression changes Signal expressed proteins Transcriptomic analysis => functions adaptation
Transcriptomic analysis High-throughput sequencing : => Higher quantity of data. => Lesser cost. Problematic: * How to store data ? * How to analyse data ?
Available tools • ESTtik : • « Expressed Sequence Tag Treatment and Investigation Kit » • Automatic transcriptomic annotation package • Can manage 454 data • Integrate the analysis in a complete database and request web interface. http://esttik.cirad.fr
454 data Transcripts Random cutting 200 – 800 bases 454 sequencing => ~380 bases Transcript contig assembly Consensus sequence