Genetic Control of Temperature Preference in the Nematode Caenorhabditis elegans

1. Genetic Control of Temperature Preference in the Nematode Caenorhabditis elegans Akiko Mohri, Elji Kodama, Koutarou Kimura, Mizuho Koike, Takafumi Mizuno and Ikue Mori

2. Background • Worms will migrate to cultivation temperature when on a temperature gradient (H&R, 1975) • Worm will avoid temperatures paired with starvation • If E. coli paired with new temperature, take 2-4 hours before worms will migrate to the new temperature

3. Neural circuit Mori 1995

4. Thermotaxis Phenotypes Isothermic Athermotactic 25˚C 25˚C 15˚C 15˚C N2 aho-2 hen-1 No ablation tax-2 tax-4 sel-12 RIA ablation Themophilic Cryophilic 25˚C 25˚C 15˚C 15˚C ttx-3 nsc-1 AFD/AIY ablation lin-11 AIZ ablation

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18. Conclusions • Respond differently to changes in temperature vs. feeding state • aho-2 defective thermotactic avoidance