1 / 4

ST-HSC

B-lineage. ST-HSC. B220 +. CLP. T-lineage. Sca-1 low cKit low IL-7R  high Flt3 high TdT +. Lin - Sca-1 high cKit + CD34 + Flt3 - Thy1.1 low. Lin - Sca-1 high cKit + CD34 - Flt3 - Thy1.1 low. CD3  + /CD8  +. MPP. Granulocyte lineage. Sca-1 - cKit + IL-7R  - FcR high

neena
Download Presentation

ST-HSC

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. B-lineage ST-HSC B220+ CLP T-lineage Sca-1low cKitlow IL-7Rhigh Flt3high TdT+ Lin- Sca-1high cKit+ CD34+ Flt3- Thy1.1low Lin- Sca-1high cKit+ CD34- Flt3- Thy1.1low CD3+/CD8+ MPP Granulocyte lineage Sca-1- cKit+ IL-7R- FcRhigh CD34+ Gr-1+ GMP Macrophage lineage LT-HSC CMP Lin- Sca-1+ cKithigh Flt3+ Mac1+ Sca-1- cKithigh IL-7R- FcRlow CD34+ Erythroid /Megakaryocyte lineages Sca-1- cKit+ IL-7R- FcRlow CD34- MEP Ter119+ HSCs HPCs Committed Lineage LSK, HSC-enriched Supplemental Figure 1

  2. c-kit 0.2% MPP LSK/MPP A. Vehicle Sca-1 Flt3 0.5% CLP IL-7Ra CLP Sca-1 Flt3 0.6% CMP IL-7Ra CMP Sca-1 FcR CD34 LSK34-/ LSK34+ 0.6% ST-HSC Thy1.1 0.3% LT-HSC Sca-1 Flt3 CD34 Supplemental Figure 2

  3. LSK/MPP c-kit 0.3% MPP B. TCDD Sca-1 Flt3 0.4% CLP CLP IL-7Ra Sca-1 Flt3 0.2% CMP CMP IL-7Ra FcR CD34 Sca-1 LSK34-/ LSK34+ 0.8% ST-HSC Thy1.1 0.2% LT-HSC Sca-1 CD34 Flt3 Supplemental Figure 2

  4. Bone marrow cells Counts (x10-6) C A % Lin- cells 8 LSK MPP 1 B % Bone marrow cells 100 Gr-1 24 CMP Ter-119 20 CLP 2 20 CD3 LSK34- 4 60 B220 2 LSK34+ Days after 5-FU treatment Days after 5-FU treatment Mac-1 30 11 16 16 11 9 0 5 3 9 0 5 3 Supplemental Figure 3

More Related