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WASTE DISPOSAL FLOWCHART

Does the waste contain any radioactive materials?. YES. YES. YES. YES. YES. NO. NO. NO. NO. NO. Does the waste contain sharps (syringes, razor blades, Pasteur pipets, microscope slides)?.

neil-richardson
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WASTE DISPOSAL FLOWCHART

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  1. Does the waste contain any radioactive materials? YES YES YES YES YES NO NO NO NO NO Does the waste contain sharps (syringes, razor blades, Pasteur pipets, microscope slides)? Does the waste contain material of human or animal origin (blood, blood products, tissues, cell lines)? Is waste contaminated with any material of human or animal origin? Does the waste contain cultures of bacteria, recombinant DNA organisms, viruses, or material that is contaminated with such agents (pipet tips, gloves, microfuge tubes, etc.)? See Autoclaving Flowchart Material can be disposed with regular trash or down the drain WASTE DISPOSAL FLOWCHART Contact Radiation Safety Officer for assistance x6771 Contact UAF Hazmat for disposal or assistance x5617 Does the waste contain any hazardous chemicals (formaldehyde, ethidium bromide, flammable liquids, etc.), or is the pH of the material <5 or >10? Contact Incinerator Supervisor for disposal x7020 Collect sharps in puncture-resistant container marked “Sharps”. If bacterial or viral agents are present, place sharps container in autoclave bag and follow Autoclaving Flowchart If no bacterial or viral agents are present, remove any “Biohazard” labels and place sharps container in box marked “Broken Glass”. Seal and dispose in regular trash.

  2. YES YES YES YES YES YES NO NO NO NO AUTOCLAVING FLOWCHART STOP! Do not autoclave these materials. Contact EHS&RM for assistance. x6771 or x5617 Do the items that you want to autoclave contain any of the following: flammable liquids (ethanol, methanol, acetone, etc.) toxic or reactive chemicals bleach radioactive isotopes animal carcasses or large blocks of tissue Autoclave for 20 min on liquid cycle. Place items in a tray and ensure that caps are not tightly sealed (containers may explode). Separate items to allow steam circulation. Is the maximum volume in any given tube or bottle <500 mL? Are you autoclaving liquids or a combination of liquids and solid items? Autoclave for 35 min on liquid cycle. NOTE: very large volumes may require longer times. Is the maximum volume in any given tube or bottle >500 mL? Are you autoclaving onlysolid items, such as new pipet tips, empty glassware, Pasteur pipets, forceps, scissors, etc.? Place items on tray and ensure that any lids are not tightly sealed. Separate objects so that steam can circulate completely around them. Autoclave for 20 minutes using a gravity cycle. Are you autoclaving solid waste in an autoclave bag, such as contaminated agar plates, gloves, tips, swabs? Place bag in bin or tray. Add 100-200 mL water to bag just prior to placing in autoclave (to generate steam within bag during cycle). Close bag loosely. Autoclave for 35-60 minutes using a gravity cycle. After cycle is finished, place bag in gray Autoclaved Waste Disposal bin next to autoclave.

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