Human Tissue Inc

1. Human Tissue Inc Part I Linda Kock Berdien Verboon Chia-Hui Yeh Marco Janssen Joost Velterop

2. Skin - Structure • Epidermis • Keratin • Dermis • Fibroblasts • Collagen • Elastin • Hypodermis • Fat

3. Skin – Temperature regulation • Sweat glands • Hairs • Vascular constriction/dilation • Shivering

4. Methods of skin replacement • Autograft • Allograft (Allogenic and Xenogenic) • Synthetic replacement • In vivo regeneration • In vitro regeneration (ex vivo)

5. TE - Techniques • Natural Matrix • Synthetic Matrix • Bilayer skin substitute • Bioreactor?

6. TE - Properties • Lack of dermal or epidermal layers (single layer grafts) • Lack of hypodermis. • Availability/ Growing time • Absence of certain cells (e.g. melanocytes) • Absence of sweat glands and hair follicles • Lacking optimal fiber arrangement • Lacking sensory functions

7. TE - Applications • Skin grafts/ tissue grafts • Victimless meat/ leather • Bone constructions • Self-adapting clothing/ furniture • Flexible structures

8. Survey - Overall Results (1)

9. Survey - Overall Results (2) • General Concerns • Sources of Tissues • Safety Issues • Necessity • Freedom of Choice • Regulations and Responsibility

10. Survey – Recommendations • Clarify questions, for example with images • Adapt goals to needs • Better specified grades • Ask for short motivations • Subdivided open questions

11. Summary • Technique is largely incomplete • Size of TE products is insufficient • People do not like controversial use of human TE tissue