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교재 : Quantitative Chemical Analysis (5th Edition, 1999) (D. C. Harris):

교재 : Quantitative Chemical Analysis (5th Edition, 1999) (D. C. Harris): 원서사용 권장 : 2 학기 교재임. 강의실 변경 : 과 424  과 B101. 연락처 : 이동수 : 2123-2641, dslee@yonsei.ac.kr ( 메일사용 권장함 ): 과 442. 조교정보. 성적평가 : 1. 수업참여도 : 10% 2. 시험 (3 회 ): 65% 3. 문제풀이 및 기타 : 25%.

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교재 : Quantitative Chemical Analysis (5th Edition, 1999) (D. C. Harris):

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  1. 교재 :QuantitativeChemicalAnalysis (5thEdition,1999) (D.C.Harris): 원서사용권장 : 2학기 교재임 강의실 변경: 과 424  과 B101

  2. 연락처:이동수:2123-2641,dslee@yonsei.ac.kr(메일사용권장함):과442연락처:이동수:2123-2641,dslee@yonsei.ac.kr(메일사용권장함):과442 조교정보 성적평가: 1. 수업참여도:10% 2.시험(3회): 65% 3.문제풀이및기타:25% 조교: 하재윤추후공고과443A,443B

  3. 화학: the science that deals with the properties, composition, and structure of substances (defined as elements and compounds), the transformations that they undergo, and the energy that is released or absorbed during these processes. 무기화학: 무기물을 관찰하여 얻은 정보(구조, 성질, 반응 등)를 분류. 정리하여 체계화 한 학문 유기화학: 유기물을 관찰하여 얻은 정보(구조, 성질, 반응 등)를 분류 정리하여 체계화 한 학문 분석화학: 화학에서 다루는 모든 대상물질의 관찰방법을 다룸 물리화학 : 무, 유기물에 대한 모든 지식과 정보들의 공통적인 배경을 귀납하고 가설을 세워 이론적인 배경을 도출하여 체계화 시킨 것

  4. 분석화학 영역 • environmental The ore-carrier Treasure sunk off the coast of South Africa June 23, 2000, spilling 1300 tons of oil. The massive oil spill oiled thousands of birds who live on Robben and Dassen Islands. • manufacturing • forensic traces of car paint found on the bicycle Chemical analysis plays an essential role in all aspects of chemistry:

  5. sleuthing 2-dimensional diffraction pattern of small amount of soil sample traces of soil on the boot • Medicine • Archeology

  6. 분석화학의 분류 정성분석과 정량분석: 정성분석: 시료에 들어 있는 원소나 화합물의 종류 정량분석: 시료에 들어 있는 원소나 화합물의 량 --> 분석화학에서는 주로 정량분석을 다룸 고전분석과 기기분석 고전분석(습식분석): 시료 중 분석물질을 분리하여 무게나 부피측정을 통해 정량 기기분석 : 분석물질의 물리적 성질(전기, 빛, 질량등) 측정을 통해 정량 두 방법 모두 유용함: 분석화학I은 주로 고전분석내용

  7. 용어 (Terminologies) Heterogeneous material composition of the material varies from one part to another Homogeneous material composition of the material is uniform throughout Lot the total material from which samples are taken from Bulk sample a portion of material obtained from the lot for analysis Laboratory sample a portion of the bulk sample

  8. Aliquot a small test portion of the laboratory sample which are used for individual analyses. Analytethe chemical substance being measured during a chemical analysis Interference occurs when species other than the analyte increases or decreases the response of the analytical method thus making it appear that there is more or less analyte than is actually present 분취량 분석물질 방해물질

  9. Masking transforming interfering species into a form that is not detected by the analytical method; eg Analysis: Ca2+ in natural waters Analytical method: measurement with EDTA 적정 Interference: dissolved Al in the water also reacts wit EDTA thus causing interference to the analysis Masking: Treatment of water sample with excess F- which reacts with Al3+ to form AlF3- which does not react with EDTA 6

  10. 화학분석의 일반절차 1. Define problem 2. Select analytical procedures 문헌 조사 ㅡ> 방법선택/ 개발 3. Bulk sampling 4. Sample preparations :분쇄, 혼합, 용액화, 농축, 분리, mask 5. Measuring analyte concentration 6. Reporting and interpretation: 신뢰도(유효 숫자), 정확도 7. Drawing conclusions: 올바른 정책결정, 반영 유도

  11. 0장 화학분석과정 (절차) Task : 쵸코렛 함유 카페인과 티오브롬 정량 티오브롬 : 카페인의 전구물질 분석방법 선택 : 고성능 액체크로마토그래피법 (HPLC:High Performance Liquid Chromatography) Chemical Abstracts search를 통해 방법 선정 HPLC : Text p 713 참조

  12. 시료제조 (Sample Preparation) • 마쇄하기 • 무게재기 • 용해-원심분리-여과로 방해물질제거 (지방, 비 용해성 물질) Fig 0-2 Fat extract, Fig 0-3 residue removal 쵸코렛의 조성 1. 분석물질 (카페인, 티오브롬) : 수용성 2. 지방(fat) : 주요방해물질 : 지용성 3. 비 용해성 물질(polysacharide?) : HPLC 분석방해 : 여과제거

  13. Chemical Analysis 액체 크로마토그래피의 원리: Fig 0-4 Using High Performance Liquid Chromatograph(HPLC) Stationary Phase : C-18 column Mobile Phase (eluant) : 79% H2O+ 20% MeOH + 1% HOAc UV detection Fig 0-5 HPLC chromatogram for the sample

  14. From Chromatogram, Peak Identification  Fig 0-6 머무름시간(retention time) 정성분석 Peak Area or Height 정량분석 Calibration Curves (검량선) See Fig. 0 -7 : Standard Preparation Interpreting the Results See Table 0-1 Analysis results Standard deviation (표준편차)

  15. 5cm radius patches chosen at random Random heterogeneous material Sampling and Sample Preparation 1. Sampling : process of selecting representative material . 비균질 시료의 채취 예1) Al정량을 위한 해수채취 (Fig. 0-8) Na나 Cl의 경우 문제가 덜 심각함 예2) glass panel (Box 0-1), or Rock

  16. 2. Sample preparation: process which converts the bulk sample into homogeneous laboratory sample; also refers to steps that eliminate interfering species. . 분쇄-혼합 Bulk sample --> Laboratory sample : Fig. 0-9 Take an aliquot for analysis . 방해물질 제거, 분석물질 농축 예1 ) 분리를 통한 Fat의 제거 예2 ) Masking법 : Ca+2 정량시 Al +3 masking by F-

  17. Lot the total material from which samples are taken from Bulk sample a portion of material obtained from the lot for analysis Laboratory sample a portion of the bulk sample Sample type Fig. 0- 9

  18. 화학분석의 일반절차 1. Define problem 2. Select analytical procedures 문헌 조사 ㅡ> 방법선택/ 개발 3. Bulk sampling 4. Sample preparations :분쇄, 혼합, 용액화, 농축, 분리, mask 5. Measuring analyte concentration 6. Reporting and interpretation: 신뢰도(유효 숫자), 정확도 7. Drawing conclusions: 올바른 정책결정, 반영 유도

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