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Toward Better Biointegration of Boston KPro

Toward Better Biointegration of Boston KPro. Kyung Jae Jeong, Ph.D., Claes H. Dohlman, M.D., Ph.D., and Daniel S. Kohane, M.D., Ph.D. Harvard-MIT Health Sciences and Technology, Department of Anesthesiology, Children’s Hospital Boston, Harvard Medical School

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Toward Better Biointegration of Boston KPro

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  1. Toward Better Biointegration of Boston KPro Kyung Jae Jeong, Ph.D., Claes H. Dohlman, M.D., Ph.D., and Daniel S. Kohane, M.D., Ph.D. Harvard-MIT Health Sciences and Technology, Department of Anesthesiology, Children’s Hospital Boston, Harvard Medical School Massachusetts Eye and Ear Infirmary, Harvard Medical School *The authors have no financial interest in the subject matter of this poster.

  2. Boston Kpro and Biointegration • Cell migration • Cell proliferation with minimal immune response • ECM Production • Adhesion • Good biointegration will also reduce the danger of bacterial/fungal infection. Khan et al. (2008)

  3. Advantages of Dopamine-Based Surface Chemistry Lee et al. Nature (2007) Lee et al. Adv. Materials (2009) Dopamine (or DOPA) is a major component of mussel adhesive protein and has good adhesive properties. Polydopamine coating can form on virtually all surfaces. Polydopamine coating can further be used to immobilize biological molecules which contain either thiol or amine groups at a high density. Chemistry is very simple.

  4. Corneal Epithelial Cells on Various Surfaces PMMA PMMA-RGD MTS assay on corneal epithelial cells Dopa-PMMA-RGD Dopa-PMMA Fluorescence micrographs of epithelial cells. Nucleus (blue) and actin cytoskeleton (red)

  5. Keratocytes on Various Surfaces PMMA-RGD PMMA MTS assay on keratocytes Dopa-PMMA Dopa-PMMA-RGD

  6. Biological Effect of Polydopamine Coating Calcium assay. on human aortic smooth muscle cells (HASMC). Red point corresponds to polydopamine coating. Higher fluorescence response corresponds to higher cell contraction. IL-6 concentration from the cells seeded on various surfaces after 24 hours. TNF- α was not detected from neither cell types. Polydopamine coating does not release dopamine monomer (mass spectrometry) Minimal contraction of the aortic smooth muscle cells (Calcium Flux Assay). Not significantly high level of proinflammatory cytokines released from the cells seeded on the polydopamine surface (ELISA, IL-6 and TNF-α).

  7. SEM Images PMMA-RGD PMMA Dopa-PMMA Dopa-RGD-PMMA

  8. Adhesion of Polydopamine Coated PMMA to Collagen Gel A small PMMA cylinder was pressed onto collagen gel over night to see if there is any adhesion at the interface. PMMA Cylinder Collagen Gel PMMA Dopa-PMMA

  9. Summary Polydopamine coating enhanced the proliferation of corneal epithelial cells and keratocytes in vitro. Polydopamine coating is not proinflammatory and is not expected to cause vascular constriction as its monomer would do. SEM images show ECM secretion from all surfaces. Both polydopamine-coated surfaces exhibited firm adhesion to collagen gel. Polydopamine coating may enhance the biointegration of Boston KPro when applied to the stem of the device.

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