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Automating the Cell Culture Sampling Process

Automating the Cell Culture Sampling Process. Mike Phipps Tara Ryan BME 273 February 11, 2002. Problem. Cell cultures maintained in bioreactors for Research and Development purposes in pharmaceutical companies must be sampled at least once daily

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Automating the Cell Culture Sampling Process

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  1. Automating the Cell Culture Sampling Process Mike Phipps Tara Ryan BME 273 February 11, 2002

  2. Problem • Cell cultures maintained in bioreactors for Research and Development purposes in pharmaceutical companies must be sampled at least once daily • methods of manually withdrawing a sample from the bioreactor can be reliable but still come with risks of culture contamination • lab workers must be trained and experienced in sterile technique

  3. Existing Sampling Methods

  4. Existing Sampling Methods

  5. Project Goals • reduce the risk of contamination that occurs due to sampling • reduce the time it takes a lab worker to draw a sample from a culture • reduce the skill and training required by a lab worker

  6. Design Ideas Idea #1 • Continuous flow of medium and cells through tubing loop • switch 3-way valve to the sampling line in order to draw a sample • simple • does not avoid the traditional syringe switch

  7. Design Ideas Idea #2 • Ethanol and wash sterilize the syringe tip (needle) • Use of septa • Expand to a set of 4 bioreactors

  8. Design Ideas Idea #3 • Open flame sterilizes the syringe tip (needle) • Use of septa • Water-gasket bioreactor system for better maintenance of the culture’s temperature • Expand to a set of 4 bioreactors

  9. Design Ideas Idea #4 • Simpler (fewer steps for mechanical arm) • Reliance on hood to provide sterility • Expand to a set of 4 bioreactors

  10. Future Work • Selecting the final design (possibly a combination of the ideas presented) • Calculations (heat transfers, air flows, etc.) to determine specifications of the final design • Draw final design using AutoCAD • Production of a prototype?

  11. References • ABEC Website, <http://www.abec.com> • B. Braun Biotech Website, <http://www.bbraunbiotech.com> • Bailey, James E., and Ollis, David F. Biochemical Engineering Fundamentals. McGraw-Hill Inc.: St. Louis, 1986. • Balcarcel, R. Robert. Associate Professor of Chemical Engineering, Vanderbilt University. • New Brunswick Scientific Website, <http://www.nbsc.com> • Todar, Kenneth. “The Control of Microbial Growth.” 21 September 2000 <http://www.bact.wisc.edu/microtextbook/ControlGrowth/sterilization.html>

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