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Leafhopper-Borne Maize Yellow Stripe Tenui-like Virus (MYSV)

Leafhopper-Borne Maize Yellow Stripe Tenui-like Virus (MYSV). History. History. 1975, Egyptian National Campaign For maize improvement has been started 1980, MYS infection has been recorded as sporadic single infected plants in maize fields of Lower Egypt

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Leafhopper-Borne Maize Yellow Stripe Tenui-like Virus (MYSV)

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  1. Leafhopper-Borne Maize Yellow Stripe Tenui-like Virus (MYSV)

  2. History

  3. History • 1975,Egyptian National Campaign • For maize improvement has been • started • 1980,MYS infection has been • recorded as sporadic single infected • plants in maize fields of Lower Egypt • 1981,MYSV has been recorded and • identified using bioassay and EM

  4. 1991, First severe outbreak caused by MYSV in maize fields of Middle Egypt (BeniSuef, Giza and Minia) 1996,MYSV incidence has been confirmed in Sudan in some sorghum-infected samples 1999,Second severe outbreak caused by MYSV in Middle and Upper Egypt maize fields

  5. 1999, MYSV partial sequencing for viral genome in INRA, France has been done (Mahmoud et al, 2000) 2000, MYSV has been proven as causal for second outbreak in maize fields in Middle (Beni Suef) andUpper Egypt (Sohag and Quena) (Aboul-Ata et al, 2007)

  6. 2004-2007, Extensive field survey in Lower and Middle Egypt aiming at identifying MYSV-outbreak-causing factors. incidence was done using MYSV polyclonal Ab and PCR for maize, weed and insect vector 2010, Third severe outbreak caused by MYSV in maize fields at Beni Suef as Middle Egypt and Sohag and Quena as Upper Egypt

  7. PROBLEM IDENTIFICATION

  8. Different MYSV Symptoms 1

  9. c a b • Insect-Inoculated • maize leaves have • Fine (a), Corse (b) stripe and Chlorotic Stunt (c) • Natural infection • Corse stripe • 2. Chlorotic 2 1

  10. Nili-growing maize as sources of MYSV infection for the coming maize plantations

  11. PCR reaction for samples 6 to 11 (bulk B) those collected from El-Fayoum and tested for MYSV Sequence of the primers used for virus detection.

  12. Epidemiology of MYSV consists of long retention Period (insect life span), up to 10 days as incubation period in the maize hosts and up to 7 days as latency in the insect vector . MYSV is not transovarially transmitted Yield reduction, of moderate MYSV infection, is as much as 63.6 % in maize and it is 70.2 % In sweet corn (Aboul-Ata and Ammar, 1988 & 1991). There are no resistant or tolerant varieties against MYSV produced yet.

  13. PROBLEM ANALYSIS

  14. Maize yellow stripe virus (MYSV) has features in common with tenuiviruses, It is transmitted in a Persistent propagative manner by a leafhopper, Cicadulina chinai (Cicadellidae,Hemiptera). purified particles (filamentous, 4–8 nm in diameter) presence of needle-shaped non-capsid protein associated with MYSV infection

  15. B A Helical structure of MYSV particles A, B and C are different Magnifications

  16. ِD ِE ِB ِA CicadulinachinaiGhuri, MYSV Insect Vector (Abdel-Kader, 2007) ِC

  17. Virus-Insect Vector Relationship

  18. Insect-Virus Relationship MYSV Persistency

  19. Effect of insect sex on transmission Effect of virus on insect fecundity

  20. Liner Relationship between MYSV Infection % and Virulefirous Insect Vector %

  21. Virus Ecology

  22. Normal incidence of MYSV in maize fields is always low in summer. It is moderate in Nily season. Outbreak of severe MYSV infection could happen each 9 years when hot winter occurred. Recently, MYSV infection is higher than normal in summer season of 2004. Change of virus ecology could lead to a severe outbreak during summer 2009

  23. Percentage of MYSV infection on Summer- and Nily-growing maize fields during outbreak and non-outbreak periods of 29 years

  24. THANK YOU ALL SO MUCH

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