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Learn about photosynthesis, a two-stage process occurring during daylight, with light-dependent and -independent reactions. Explore the evidence, including the impact of temperature, and delve into the chemistry involved in this essential natural process.
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Photosynthesis Two-stage process Light-dependent reaction Occurs only during daylight Light-independent reaction May continue in the dark
Photosynth • https://www.youtube.com/watch?v=g78utcLQrJ4 • Respiration • https://www.youtube.com/watch?v=0IJMRsTcwcg
Evidence for two stages Temperature has a clear effect on the rate of photosynthesis This suggests that although light levels initially limit the rate, there is a second stage controlled by temperature-sensitive enzymes A plant given alternating periods of dark and light forms more carbohydrate than one in continuous light Period of darkness ensures that all the product from first stage is converted into carbohydrate before it builds up
Excitation of electrons Photon of light hits chlorophyll molecule Energy transferred to the electrons in the molecule Electrons raised to higher energy levels If they are raised to a high enough level they will dissociate Electrons picked up by an electron acceptor ATP formed as the electron is passed along an electron transport chain
Electron transport chain electron acceptor ATP ADP + Pi final electron acceptor
Light-dependent stage Occurs in the thylakoids Involves splitting of water by light – photolysis ADP is converted to ATP by light – photophosphorylation Cyclic photophosphorylation uses only PSI Non-cyclic photophosphorylation uses both PSI and PSII
Cyclic photophosphorylation e- electron acceptor ATP ADP + Pi Chlorophyll e- light
Non-cyclic photophosphorylation electron acceptor e- 2H ATP electron acceptor e- ADP + Pi 2H+ e- Photosystem I Photosystem II H2O e- light light ½O2
NADP NADP+ e- NADPH + H+ 2H To the light independent reaction 2H+
Photolysis of water 4 H2O → 4 H+ + 4 OH- 4 OH- - 4 e- → O2 + 2 H2O Splitting of water is catalysed by an enzyme – perhaps PSII! NADP+ Photosystem II
Light-dependent stage Water is converted to protons, electrons and oxygen Reduced NADP is generated Occurs in the thylakoid membrane
Non-cyclic photophosphorylation electron acceptor e- 2H ATP electron acceptor e- ADP + Pi 2H+ e- Photosystem I Photosystem II H2O e- light light ½O2
NADP NADP+ e- NADPH + H+ 2H To the light independent reaction 2H+
Melvin Calvin Member of the Radiation Laboratory at Berkeley, University of California Studied using radioactively labelled carbon dioxide 14CO2 fed to Chlorella algae and its path tracked
Light-independent stage Carbon dioxide is converted to carbohydrate Occurs in the stroma of the chloroplast
Three phases Carboxylation Carbon dioxide fixation with ribulose bisphosphate (RuBP) Reduction Reduction of glycerate 3-phosphate (GP) to glyceraldehyde 3-phosphate (GALP) Regeneration Re-formation of the CO2 acceptor molecules
Phase 1: Carboxylation Rubisco CO2 GP 6C molecule RuBP GP
Phase 2: Reduction 2ATP 2ADP + 2Pi GP GALP GALP GP 2NADPH + H+ 2NADP+
Phase 3: Regeneration ATP ADP + Pi GALP RuBP GALP
Synthesis of Organic Substances GALP GP GALP GALP GALP fatty acids glycerol amino acids hexose lipids
The Maths 2 molecules of GALP are made each turn 5 out of 6 molecules of GALP are used to regenerate RuBP 2 molecules of GALP are needed to make a hexose sugar How many turns are needed to make 1 molecule of hexose sugar? How many molecules of ATP and reduced NADP are needed?