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Construction of liver-specific PERK transgenic vector

Construction of liver-specific PERK transgenic vector. Why liver specific transgene ?. Alb-aFP-Cre. Kellendonk C, et al. Hepatocyte-specific expression of Cre recombinase. Genesis. 2000;26:151-3. Alb-aFP vector. From Dr. Kellendonk C @ Columbia University.

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Construction of liver-specific PERK transgenic vector

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  1. Construction of liver-specific PERK transgenic vector

  2. Why liver specific transgene ?

  3. Alb-aFP-Cre Kellendonk C, et al. Hepatocyte-specific expression of Cre recombinase. Genesis. 2000;26:151-3.

  4. Alb-aFP vector From Dr. Kellendonk C @ Columbia University

  5. Alb-aFP-PERK construction strategy PCR cloning of PERK with XhoI sites at both ends. Template is PERK-c-myc from David Ron. Modified version of Pfu DNA polymerase.

  6. Alb-aFP-PERK construction strategy mPERK-9E10

  7. What’s next ? Sequence full length. Test in HepG2 cell culture. Microinject male pronuclei

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