1 / 13

MICROBIOLOGY LABWORK JPEMS 2012 NANTES

MICROBIOLOGY LABWORK JPEMS 2012 NANTES. Dr C. Bressollette (Virology, Nantes) Dr J. Caillon (Bacteriology, Nantes) Dr M. Eveillard (Bacteriology, Angers) Cécile Peltier (Virology, Nantes). The week schedule. Virology Viral cultures: Observation of a cytopathic effect

nolan-moore
Download Presentation

MICROBIOLOGY LABWORK JPEMS 2012 NANTES

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. MICROBIOLOGY LABWORKJPEMS 2012NANTES Dr C. Bressollette (Virology, Nantes) Dr J. Caillon (Bacteriology, Nantes) Dr M. Eveillard (Bacteriology, Angers) Cécile Peltier (Virology, Nantes)

  2. The week schedule • Virology • Viral cultures: • Observation of a cytopathic effect • HSV typing by immunofluorescence • Molecular assay • Amplification of a viral gene • Serologic assay: • Inhibition of hemagglutination • Bacteriology • Analysis of a bacterial mixture • Gram staining • Bacterial identification • Susceptibility to antibiotics • Analysis of the environment

  3. Day 2 – Viral cultures • Observation of non infected adherent cells under the microscope Human fibroblasts Primary cells, limited multiplication in vitro HEP-2: epithelial cells, immortalized cell line

  4. Day 2 – Viral cultures • Observation of infected adherent cells under the microscope Enterovirus Respiratory Syncitial Virus

  5. Day 2 – Viral cultures • Observation of infected adherent cells under the microscope HSV Adenovirus

  6. Day 2 - Virology • Observation of infected adherent cells under the microscope Cytomegalovirus

  7. Day 2 – Virology IHA

  8. Day 2 – Virology PCR

  9. Day 2 - Bacteriology • Analysis of the bacterial mixA, B, C, D ,E : Observe the aspect of the colonies on the 3 agar plates • Chromogenic agar plate : 2 sorts of colonies • Chapman : only 1 type of colonies • Drigalski : only 1 type of colonies • lactose positive (yellow colonies) • or lactose negative (blue-green colonies) according to the student pairs

  10. Day 2 - Bacteriology • Biochemical identification of the colonies isolated on the Drigalski agar plate • Seed an identification gallery • Study the biochemical characters of the bacteria • See the demonstration and the lab work workbook

  11. Bacterial identification Api system Scratch 2 or 3 colonies from the agar plate Rub on the glass wall and moisten progressively Sterile distilled water Not underlined Not framed Just fill the tubule with the suspension Cup Tubule Underlined Cover with oil in the cup Framed: fill completely (tubule + cup)

  12. Antibiogram by disk diffusion Principle the antibiotic disk gets wet in contact with the agar It diffuses into the gel, with a decreasing gradient of concentration from the disk to the periphery. Method • - Prepare a bacterial suspension from a colony with a sterile loopful, in a tube of sterile distilled water; • Dip the swab in the suspension • Inoculate the agar plate with close streaks,

  13. Antibiogram by disk diffusion Method Place the antibiotic disks immediately.

More Related