Consensus

1. Consensus M A A E A D - X D - - E A - - - - - - - HSV1 UL26 M A A D A P - G D R M E E - - - - - P L 14 VZV ORF33 M A A E A D - E E N C E A - - - - - - - 12 CMV UL80 M T M D E Q - Q S Q A V A P V Y V G - - 17 FPTHV UL26 M Q T E E D - A P - - - - - - - - - - - 8 LETV UL26 M A A E T S Y P D - - - - - - - - - - - 9 Consensus - - - - - - L Y V A G F L A L Y D - - X HSV1 UL26 P D R A V P I Y V A G F L A L Y D - - S 32 VZV ORF33 - - - - - - L Y V A G Y L A L Y S - - K 24 - - - - Supplemental Data Table 2:Alignment of the N-terminal domains of the UL26 homologs of herpes simplex virus 1 (HSV1), human herpesvirus 3 (Varicella-Zoster virus, VZV), human herpesvirus 5 (cytomegalovirus, CMV), FPTHV, and LETV. Highly conserved histidine residues (Liu&Roizman, PNAS 89:2076) are in bold. CMV UL80 - - G F L A R Y D Q S P D - - E 29 FPTHV UL26 - - - - - - L Y V A G F L G L Y D R G G 22 LETV UL26 - - - - - - V Y V G G F L A V Y E - - A 21 Consensus - D E G E L X L D - - - - - - R E I V R HSV1 UL26 G D S G E L A L D - - - - - - P D T V R 46 VZV ORF33 - D E G E L N I T - - - - - - P E I V R 37 CMV UL80 - A E L L L P R D V V E H W L H A Q G Q 48 FPTHV UL26 - E E R G F R L D - - - - - - R E A V R 35 LETV UL26 - D V S E L F L N - - - - - - R E I M A 34

2. Consensus R A L P L T S X V P L N I D H R A G C V HSV1 UL26 A A L P P D N P L P I N V D H R A G C E 66 VZV ORF33 S A L P P T S K I P I N I D H R K D C V 57 CMV UL80 G Q P S L S V A L P L N I N H D D T A V 68 FPTHV UL26 R G L P L T Q I V P L C I D H K D R C T 55 LETV UL26 R A L P T D S V V P L N I D H R A G C V 54 Consensus V G R V - A X V X D X R G G L F C L G K HSV1 UL26 V G R V L A V V D D P R G P F F - V G L 85 VZV ORF33 V G E V I A I I E D I R G P F F - L G I 76 V G H V CMV UL80 - A A M Q S V R D G L F C L G C 87 FPTHV UL26 V G R V - L S V L D A E K G A F C V A K 74 LETV UL26 V G R V - L T I H N G R Y G L F C L C K 73 Consensus V T S P N L F R V L X E A A D X X X F E HSV1 UL26 I A C V Q L E R V L E T A A S A A I F E 105 VZV ORF33 V R C P Q L H A V L F E A A H S N F F G 96 CMV UL80 V T S P R F L E I V R R A S E K S E L V 107 FPTHV UL26 V T S K N L F R I M L E T V D P R V F E 94 LETV UL26 I T S Q N L F R V M M E T A D E G L F E 93

3. Consensus N R G P G - - L S E X E K L L Y L I S N HSV1 UL26 R R G P P - - L S R E E R L L Y L I T N 123 VZV ORF33 N R D S V - - L S P L E R A L Y L V T N 114 CMV UL80 S R G P V S P L Q P D K V V E F L S G S 127 FPTHV UL26 - L K P G - - L S E R E K L L L L I S N 111 LETV UL26 N L N P G - - - S E A D K L L Y L I S N 110 Consensus Y L P S V S L S S K R - - - - - - V X S HSV1 UL26 Y L P S V S L A T K R - - - - - - L G G 137 VZV ORF33 Y L P S V S L S S K R - - - - - - L S P 128 Y - A G CMV UL80 L S L S S R R C D D V E A A T S 146 FPTHV UL26 F V P S F S L S S V K - - - - - - V D R 125 LETV UL26 Y I P S F S M S S K R - - - - - - V A S 124 Consensus X E E P D X T F F X H V A L C X V G R R HSV1 UL26 E A H P D R T L F A H V A L C A I G R R 157 VZV ORF33 N E I P D G N F F T H V A L C V V G R R 148 CMV UL80 L S G S E T T P F K H V A L C S V G R R 166 FPTHV UL26 P E E A D E R F F V H V S L C M L G R R 145 LETV UL26 V D E V D S T F V S H V S L C I L G K R 144