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Digital Biology As investigated by NANECTIS Biotechnologies SA 2007 Paris & New-York

Digital Biology As investigated by NANECTIS Biotechnologies SA 2007 Paris & New-York. I - FACTS.

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Digital Biology As investigated by NANECTIS Biotechnologies SA 2007 Paris & New-York

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  1. Digital BiologyAs investigated byNANECTISBiotechnologies SA2007 Paris & New-York

  2. I - FACTS Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

  3. Capture of the signals Signal Analysis software Sample X 500 Sensor coil Computer Amplifier

  4. synoptic signal source virtual instrument analog board direction rotation angle current presence variation of magnetic field signal conditioning & acquisition ampli low noise sensor server measurement system storage on-offline work nanectis 2007 alain cazals

  5. sensor • mu-métal shield • high sensibility • good signal/noise with mu-métal shield • good linearity • spec : 20µV - 292Ω - 126mH • possibility dual-mode nanectis 2007 alain cazals

  6. amplification • double input/output • gain : 50dB - 60dB - 70dB • bandwith : 50Hz to 50kHz at 70 dB • distortion : 0,04% at -10 dBV 0,12% at OdBV • very low noise : -124dBV (0,63µVrms) • transistor amplifier stage nanectis 2007 alain cazals

  7. filter & acquisition • virtual instrument managing with LabView suite software • automatic stopband filter (follow fS) • master timebase : 12.8MHz4 analog input channels • data rate 50kS/sADC resolution 24 bits • low noise (70 µVrms)CMRR 126dB nanectis 2007 alain cazals

  8. signal processing • NI LabVIEW easy-to-use graphical development environment • tight integration with a wide range of measurement hardware • extensive signal processing, analysis and math functionality • multiple communication options (TCP/IP, serial) • time-series analysis :statistical analysis for description, explanation, prediction • time-frequency analysis :analytical, graphical tools for signals with evolving frequency content nanectis 2007 alain cazals

  9. Analysis of the electromagnetic signals (EMS) Amplitude 1-20,000 Hertz Time (sec)

  10. Noise Amplitude (+) Time (sec)

  11. Spectral Frequency Analysis Fourier Tranformation • A positive signal is defined by: • amplitude increase • Shift to higher frequencies (500-2000 Hertz)

  12. Paris 2006 (or Milan)

  13. Paris 2006 (or Milan)

  14. Afrique Centrale

  15. Afrique Centrale

  16. Noise (+) Time (sec) Frequency (1-20000 Hertz)

  17. Noise (+)

  18. General (empirical) rules: • a) Filtration by pore size smaller than the normal size of the micro-organism is essential • 100nM filter for bacteria • 100nM and 20nM filter for mycoplasma, MLO and viruses.

  19. General (empirical) rules: b) Signals are only detected at certain dilutions in water Amplitude N.D. 10-2 10-14 10-4 10-6 10-8 10-10 10-12 Dilutions c) Negative lower dilutions contain inhibitors of signals in positive dilutions.

  20. MycoND Myco-1 Myco-2 Myco-3 Myco-4 Myco-5 Myco-6 Myco-7 Myco-8 Myco-9 Myco-10 Myco-11 N.D. 10-2 10-14 10-4 10-6 10-8 10-10 10-12 Myco-12 Myco-13 Myco-14 Myco-15

  21. List of micro-organisms at the origin of EMS (as of January 2006) Filtrates from cultures of E.coli Streptococcus Staphylococcus Pseudomonas Mycoplasma pirum Viruses: HIV1 Influenza group A (dilution up to 10-20)

  22. Filtrates from plasmas or patients presenting with: Ureaplasma ureolyticum urethritis HIV/AIDS Rhumatoid arthritis Parkinson disease Various myo-neuropathies Cat infected with FeLV No signals ( over noise) have been detected: in the cultures of uninfected cell lines, nor in culture of Candida albicans nor in the plasma of healthy individuals or of patients suffering of hypertension, capillary fragility, diabetes, arthrosis, lung cancer. (dilution up to 10-15)

  23. The case of HIV/AIDS (in collaboration with CIRBA, Ivory Coast) African patients, generally infected with the A/G HIV1 subtype (in vitro : CEM cells infected with HIV1 Lai) F D-5 (-) F D-7 (+) F D-8 (+) F D-9 (-)

  24. Plasma from a seropositive patient under HAART with undetectable virus load

  25. E. Coli EMS (+) between 10-9 and 10-18 F D-8 (-) F D-9 (+) F D-10 (+) F D-19 (-)

  26. EMS After filtration 0,45µ then 0,1µ Dilutions in water

  27. Influenza A From cultures of MDCK cells NF F D-2 F D-8 (-) F D-9 (+) F D-3 F D-4 F D-10 (+) F D-19 (-) (in coll. with the Laboratoire de Santé Publique du Quebec)

  28. Plasma from patient with Alzheimer Disease EMS (+) between 10-6 and 10-11 D-5 (-) NF D-6 (+) F D-2 F D-8 (-) F D-9 (+) F D-3 F D-4 D-7 (+) F D-10 (+) F D-19 (-) D-8 (+)

  29. Plasma from patient with Alzheimer Disease NF F D-2 Dilution 10-2 negative F D-8 (-) F D-9 (+) F D-3 F D-4 Dilution 10-8 positive F D-10 (+) F D-19 (-)

  30. Plasma from patient with Rhumatoïd Arthritis F D-3 (-) F D-5 (+) F D-8 (-) F D-9 (+) F D-6(+) F D-9 (-) F D-10 (+) F D-19 (-)

  31. II - INTERPRETATION Properties of the structures in water at the origin of EMS (1) • The ULF signals are produced in water by nanostructures (naneons) whose size is proportional to that of the micro-organism from which they are derived. • a) For classical bacteria, between 100 and 20 nM • (retained by 20nM, not retained by 100nM filters) • b) For mycoplasma, L forms and large viruses: naneons are present in both 100nM and 20nM filtrates ( more in 20nM filtrates for viruses) • c) For small viruses ( which pass through 100nM filters): naneons are only present on 20 nM filters • Their titer is calculated from the highest positive dilution.

  32. 100 nM 20 nM

  33. Properties of the structures in water at the origin of EMS (2) • Naneons are resistant to :Dnase Rnase Proteinase K Triton X100 EDTA • Naneons are sensitive to: • Heat (70°C) • Freezing at -20°C • and -60°C • Slow decay with time • Their density, as measured by sucrose equilibrium density gradient, is distributed in a whole spectrum ranging from 1,0 to 1,28 with peak between 1,16 and 1,23

  34. Naneonsare clusters of water molecules in a highly dynamic state but emitting stable radio waves. Role of ions ? High density: condensed matter ? From « New insights into the Structure of Water with Ultrafast Probes By Yan Zubavicus and Michael Grunze, Science, 14 may 2004, vol 304 »

  35. Application to Diagnostic • And • Monitoring of Therapies • Blood safety • Prevention of nosocomial diseases • Detection of microbial agents in chronic diseases • Neurodegenerative diseases and psychiatric • Arthritis • Cardiovascular • Cancer • Biomarker of HIV reservoir which remains after tritherapy

  36. “Absence of evidence is not evidence of absence” Carl Sagan

  37. Pr Luc Montagnier Scientific Collaborators: Jamal Aissa……………………………Paris Claude Lavallée………………….New-York René Olivier……………………………Paris

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