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M. Willrich Siebert, L. B. Braxton, R. M. Huckaba, L.C. Walton, R.A. Haygood,

Field Performance of WideStrike Insect Protection Against Key Lepidopteran Pests in the Mid-South And Southeastern U.S. TM. M. Willrich Siebert, L. B. Braxton, R. M. Huckaba, L.C. Walton, R.A. Haygood, R.B. Lassiter, F. Haile, and G. D. Thompson. * Dow AgroSciences LLC., Indianapolis, IN.

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M. Willrich Siebert, L. B. Braxton, R. M. Huckaba, L.C. Walton, R.A. Haygood,

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  1. Field Performance of WideStrike Insect ProtectionAgainst Key Lepidopteran Pests in the Mid-South And Southeastern U.S. TM M. Willrich Siebert, L. B. Braxton, R. M. Huckaba, L.C. Walton, R.A. Haygood, R.B. Lassiter, F. Haile, and G. D. Thompson *Dow AgroSciences LLC., Indianapolis, IN TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  2. Heliothine Complex In Cotton • Bollworm, Helicoverpa zea • Tobacco Budworm, Heliothis virescens • Mid-south and southeastern U.S. • 2005 Cotton Insect Loss Estimates: • - Yield Reduction: 1.2% (10 states, 1 to 2.5%) • - Applications/Acre: 0.8 (0.2 to 1.5) TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  3. Secondary Lepidopteran Pests In Cotton Fall Armyworm, Beet Armyworm, Southern Armyworm Soybean Looper/Cabbage Looper • 2005 • Applications per acre: <1 • Fall armyworm: Greatest losses among secondary Lepidoptera TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  4. Cry 1Ac + Cry 1F WideStrike : A Dual-Gene Bt Technology For Broad Spectrum Lepidopteran Management TM Commercially Available In PhytoGen Varieties Since 2005 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  5. Historical Background: 2001-2005 • WideStrikeTM reduced feeding damage by tobacco budworm and bollworm to low levels. • Cry1Ac is highly effective against tobacco budworm. • WideStrikeTM sustained low-level damage under high bollworm pressure • WideStrikeTM non-sprayed yields were numerically similar if not statistically greater than the sprayed non-Bt in natural infestation tests. • WideStrikeTM provided excellent control of many of the secondary foliar feeding lepidopterous insects including cabbage looper, soybean looper, southern armyworm, and fall armyworm. TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  6. Materials And Methods • WideStrike Evaluated in 23 Locations During 2006 - No Insecticide Sprays Targeting Lepidopteran Pests - Natural Infestations • Experimental Design: Randomized Complete Block, 4 replications TM • Variables Measured: Heliothine Damage And Live Larvae, and Lint Yield • Statistical Analysis: ANOVA, α = 0.10 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  7. WideStrike Trial Locations: 2006 TM * * * * Heliothine Infestations * High * * * * * * * * * * Moderate * * * * * * * * * Low * * TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  8. PHY370WR PHY485WRF PHY310R PHY425RF Efficacy of WideStrike Against Bollworm,Rocky Mount, NC, Dr. J.R. Bradley, NCSU TM a a a a a a a a b b b b b b b b 8 Aug 24 Aug 15 Aug 8 Aug Square Damage Boll Damage Columns within a date followed by the same letter are not significantly different, P>0.10 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  9. PHY370WR PHY480WR PHY485WRF PHY310R PHY425RF PHY470WR b b b b Performance of WideStrike Against Bollworm,East Bernard, TX, Gregory Visoski Consulting Entomologist TM a a a a a a a a 2 July 7 Aug 12 July 2 July Square Damage Boll Damage Columns within a date followed by the same letter are not significantly different, P>0.10 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  10. PHY370WR PHY485WRF PHY310R PHY425RF PHY470WR PHY480WR a ab b b c c b Yield of WideStrike Varieties Exposed to High Bollworm Pressure, East Bernard, TX, Gregory Visoski Consulting Entomologist TM Columns within a date followed by the same letter are not significantly different, P>0.10 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  11. PHY370WR PHY485WRF PHY310R PHY425RF PHY470WR PHY480WR b b b b Performance of WideStrike Against Bollworm,Bossier City, LA, Steve Micinski, LSU AgCenter TM a a a a a a a a 0 0 0 0 0 0 25 July 18 July 1 Aug 11 July Columns within a date followed by the same letter are not significantly different, P>0.10 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  12. PHY370WR PHY480WR PHY470WR PHY425RF PHY485WRF PHY310R Yields of WideStrike Varieties Exposed ToSustained Bollworm Infestations, Bossier City, LASteve Micinski, LSU AgCenter TM a a a a b b b Columns within a date followed by the same letter are not significantly different, P>0.10 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  13. PHY370WR PHY425RF PHY485WRF PHY310R Efficacy of WideStrike Against Mixed Bollworm andFall Armyworm Infestations, Lonoke, AR TM Dr. Merritt Holman, Arkansas Crop Technology Square Damage (%) Boll Damage (%) 9 Aug 26 Jul 2 Aug 23 Aug 30 Aug 9 Aug 26 Jul 2 Aug 23 Aug 30 Aug *Fall armyworm infestations occurred from 9-30 Aug. TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  14. PHY370WR PHY485WRF PHY310R PHY425RF WideStrike Provides Excellent Control Of Fall Armyworm, Lonoke, AR Dr. Merritt Holman, Arkansas Crop Technology TM 23 Aug 9 Aug 30 Aug *Number per 40 bolls sampled per date. TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  15. PHY370WR PHY485WRF PHY310R PHY425RF Yields of WideStrike Varieties Exposed To Sustained Bollworm and Fall Armyworm Infestations, Lonoke, AR TM Dr. Merritt Holman, Arkansas Crop Technology a a b b c c Columns within a date followed by the same letter are not significantly different, P>0.10 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  16. Efficacy of WideStrike Against High-Level Soybean Looper Infestations, Tillar, ARDr. Charlie Guy, G&H Associates, Inc. TM a a b b Columns within a date followed by the same letter are not significantly different, P>0.10 TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  17. Conclusions • Efficacy of WideStrike™ cotton against Heliothines in 2006 similar to 2001-2005 results. • Varieties containing WideStrike provided control of Heliothis (primarily bollworm) that were statistically similar. - Boll damage exceeded 5% at four of 23 locations, all under heavy BW pressure. - Under high insect occurrence, a few locations exceeded insect threshold levels of feeding damage or larval infestation in Widestrike™ cotton – treatment necessary to prevent losses from bollworm. TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  18. Conclusions • Varieties containing WideStrike provided control of secondary lepidopteran pests that were statistically similar. TM • WideStrike effectively protected against yield loss. • Numerical and even statistic differences did occur among WideStrike varieties in one location. Yield potential and environmental conditions are likely contributing factors, rather than in-season differences in efficacy. TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

  19. WideStrike Insect Protection:Delivery of Two Insect Control Genes TM • Excellent Efficacy Against Heliothines • Control Of Key Secondary Lepidopteran Pests • Overall Reduction In Lepidopteran Sprays • Expressed In High Yielding PhytoGen Varieties TM Trademark of Dow AgroSciences LLC. TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

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