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Setting up a trinocular compound microscope for best viewing

Setting up a trinocular compound microscope for best viewing. Ocular tube for mounting a camera. Oculars for viewing by eye. “Trinocular” means three oculars, or eyepieces. Rotating turret to hold objective lenses. Objective lenses.

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Setting up a trinocular compound microscope for best viewing

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  1. Setting up a trinocular compound microscope for best viewing

  2. Ocular tube for mounting a camera Oculars for viewing by eye “Trinocular” means three oculars, or eyepieces

  3. Rotating turret to hold objective lenses Objective lenses “Compound” means having multiple objective (lower) lenses mounted on a rotating turret (lens holder)

  4. Focusing the microscope on specimen slides can be accomplished with a coarse adjustment control for rapid adjustment, and a fine control for small adjustments, particularly necessary at high magnification levels. Coarse control Fine control

  5. The “mechanical stage” holds specimen slides and allows them to be moved precisely under objective lenses to focus on any area of interest. Stage positioning controls

  6. The microscope illuminator, or light source, provides light for transmission through the specimen for viewing its structure. The amount of light is modified with a shutter or diaphragm using a control knob to open or close it for more or less light. Diaphragm control Illuminator

  7. The condenser focuses light from the illuminator onto the specimen slide to provide the best lighting for viewing. It can be raised or lowered and centered for precise focusing as necessary. It has its own shutter diaphragm too, for varying the amount of light reaching the specimen slide. Centering controls Diaphragm control Focusing knobs

  8. To send light from the microscope to a camera mounted on the third ocular tube, a diverter-prism is moved into the field of view by pulling out its control arm.

  9. Setting up a microscope for best viewing involves adjusting the parts of the scope to the viewer, focusing the scope on the specimen slide and adjusting the illuminator or light source to the slide being viewed.

  10. Turn on microscope illuminator by rotating dimmer knob slowly clockwise. Fast turn-on shortens bulb life. Dimmer knob

  11. Place a sample slide on the microscope stage under the lowest power objective lens.

  12. Adjust the microscope eyepieces so you can comfortably see a well-focused image through both Space eyepieces so you can comfortably see through both at the same time. If one eyepiece tube is fixed focus, close the opposite eye and, using the microscope focusing knob, carefully focus an image of the sample for the open eye. (If both eyepiece tubes are variable focus, either one will do.) Now close the other eye and use the variable focus eyepiece tube to bring the image into focus for the open eye.

  13. Focus on the specimen slide (e.g. these wood cells from a piece of paper). Using the 4X objective lens, there will be a much larger field of view but much less detail visible than with the 40X lens. 4X 40X

  14. Rotate the illuminator diaphragm control until the diaphragm is closed all the way. Using the focusing knob on the condenser, bring the diaphragm image into as sharp focus as possible. Using the centering controls, be sure the diaphragm image is positioned in the center of the field of view. 4X 40X

  15. Adjust the condenser diaphragm so that the specimen features are as clearly visible as desired. Condenser diaphragm wide open Condenser diaphragm closed down to provide correct contrast for clear viewing

  16. The nature of light and lenses results in a plane of focus which becomes thinner as magnification increases. That means for a particular specimen slide, it becomes possible to focus through the thickness of a specimen. Some parts will be in focus while others will not, as in these wood cells focused at different “depths” through the slide. Compare

  17. When the microscope has been adjusted to produce the best image of a specimen, it is ready for capturing with the camera. Now the diverter-prism must be pulled into position to direct light up the third ocular tube to the camera mounted on it. REMEMBER What you see in the camera or on the image-capture viewer is what will be captured to a digital file. NOT NECESSARILY what you see by eye through the eyepieces. BE SURE a final focus is made in the camera viewer or on the image-capture viewer.

  18. REVIEW • Turn on microscope illuminator slowly. • Place specimen slide on mechanical stage • Focus on specimen at desired magnification • Adjust eyepieces for best viewing • Adjust condenser for best lighting • Adjust condenser diaphragm for best contrast • Refine focus for depth of field • Refine focus for image capture • Capture image

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