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Metabolic engineering of alkaloids production in plant

Metabolic engineering of alkaloids production in plant. Juan Pan Department of Plant Pathology. outline. alkaloids biosynthesis in plant metabolic engineering summary. Alkaloids. N-containing small molecule Amino acid derivative S econdary metabolites Defense in plant

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Metabolic engineering of alkaloids production in plant

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  1. Metabolic engineering of alkaloids production in plant Juan Pan Department of Plant Pathology

  2. outline • alkaloids biosynthesis in plant • metabolic engineering • summary

  3. Alkaloids • N-containing small molecule • Amino acid derivative • Secondary metabolites • Defense in plant • Pharmaceutical uses

  4. Alkaloid biosynthesis • In plant • monoterpeneindole • alkaloid (MIA) Annu. Rev. Plant Biol. 2008. 59:735-69

  5. MIA biosynthesis scheme

  6. Alkaloid biosynthesis in plant • benzylisoquinoline alkaloids (BIAs) dopamine tyrosine (S)-reticuline (S)-norcoclaurine 4-hydroxyphenyl acetaldehyde Nature chemical biology, 2009, 5 (5): 292-300

  7. Alkaloid biosynthesis in plant • tropane alkaloids

  8. Alkaloid biosynthesis in plant • purine alkaloids

  9. Metabolic engineering of alkaloid biosynthesis • Overexpressing rate- limiting genes • Gene silencing to block pathway • Regulatory element

  10. PMT and H6H overexpression increased scopolamine production H6H Proc NatlAcadSciUSA 101: 6786-6791

  11. Silencing COR blocked morphine biosynthetic pathway COR dopamine tyrosine (S)-reticuline (S)-norcoclaurine 4-hydroxyphenyl acetaldehyde Nat Biotechnol22: 1559-1566

  12. Nat Biotechnol22: 1559-1566

  13. Exogenous regulatory factors increased alkaloids production in poppy Plant Biotechnology Journal (2008) 6, pp. 160–175

  14. Summary • Overexpression of rate-limiting enzyme for higher alkaloid production • Gene silencing to block certain pathway • Manipulating regulatory elements to increase alkaloid production • Combination of several methods

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