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Animal Cell Culture 鄭智美 Lecture Website cheng.dlearn.kmu.tw

Animal Cell Culture 鄭智美 Lecture Website http://cheng.dlearn.kmu.edu.tw N931. 課程大綱 介紹動物組織學之之生物原理 ，發展演進歷史，以及細胞培養在現代生物科技，組織工程學以及融合瘤免疫技術，基因遺傳工程組織器官移植之臨床醫療，及臨床診斷上上之應用上所扮演之角色. Mammalian Cell Culture. What is cell culture, exactly?.

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Animal Cell Culture 鄭智美 Lecture Website cheng.dlearn.kmu.tw

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  1. Animal Cell Culture 鄭智美 Lecture Website http://cheng.dlearn.kmu.edu.tw N931

  2. 課程大綱 介紹動物組織學之之生物原理 ，發展演進歷史，以及細胞培養在現代生物科技，組織工程學以及融合瘤免疫技術，基因遺傳工程組織器官移植之臨床醫療，及臨床診斷上上之應用上所扮演之角色

  3. Mammalian Cell Culture

  4. What is cell culture, exactly? • Cells, previously growing in a human or animal modified to grow in plastic or glass • In the body = in vivo • On plastic or glass = in vitro • Kept in an incubator to stay at body temperature • Use special media with nutrients so the cells can grow and divide

  5. If we treat the cells right • They might act similar to those in vivo • Making the same proteins • Then scientists can change the environment

  6. Animals are complex • Many different cells • Many different proteins • Interacting continuously • Difficult to watch individual events in vivo • Animals are usually harmed to observe biological events

  7. Endothelial cells http://www.youtube.com/watch?v=rdayl3wKiU4&feature=related http://www.youtube.com/watch?v=MY-zcuYB0_w&feature=related Epithelial cells http://www.youtube.com/watch?v=Hm03rCUODqg&feature=fvwrel Breast cancer cells Microtubule live image http://www.youtube.com/watch?v=DA8DvDE_nZo&feature=related

  8. Text Books Culture of Cells for Tissue Engineering, Freshney, RA, Wileyliss,2007 Culture of Animal Cells, 2005 ,Freshney, RA etal Culture of human stem cells , 2007, Freshney, RA ……………… and more

  9. 每週需按時上課 ， 期末依分組於規定時間及地點每組進行口頭報告，請假需按學校規定 期中考 佔學期成績 30% 期末考 佔學期成績 30% 平時成積 佔學期成績 20% 口頭報告 佔學期成績 20%

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