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whole cell lysates. IP: Trx. p54. p54. phospho-JNK. p46. p46. ASK-1. total JNK. Trx. DMSO 1. DMSO 1. DMSO 2. DMSO 2. 5 m M AJM 290. 5 m M AJM 290. 5 m M AW 464. 5 m M AW 464. actin. DMSO 0.5 1 5 10.
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whole cell lysates IP: Trx p54 p54 phospho-JNK p46 p46 ASK-1 total JNK Trx DMSO 1 DMSO 1 DMSO 2 DMSO 2 5mM AJM 290 5mM AJM 290 5mM AW 464 5mM AW 464 actin DMSO 0.5 1 5 10 0 5 30 60 180 180 min DMSO 3h AW 464 (mM) AW 464 (5mM) AW 464 induced ASK-1/Trx dissociation and JNK activation in HCT116 cells.