Witzel et al. J Cell Biol . (2006)

1. Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane Witzel et al. J Cell Biol. (2006)

2. Vertebrate Noncanonical Wnt Signaling PCP signaling play key roles in cell polarization and migration during gastrulation. Veeman et al. Dev Cell. (2003)

3. Gastrulation movements in zebrafish Solnica-Krezel L. Curr Opin Genet Dev. (2006)

4. Noncanonical Wnts and Fz regulates cell adhesion Ectopic expression of Xwnt-5A leads to decreased Ca 2+-dependent cell adhesion. Torres et al. J Cell Biol. (1996) Frizzled7 interact with protocadherin which mediate selective cell–cell adhesion. Medina et al. EMBO J. (2004) Wnt11 controls tissue morphogenesis by modulating E-cadherin-mediated cell cohesion. Ulrich et al. Dev. Cell. (2005)

5. Wnt11 induces local accumulation of Fz7 at the plasma membrane 10pg mRNA 20pg mRNA 5pg mRNA Lyn : membrane-bound kinase - membrane marker

6. Wnt11 locally accumulates with Fz7 at the plasma membrane Wnt11 producing cell Wnt11 receiving cell

7. Wnt11 induces the accumulation of Fz7 on both cells. Wnt11-induced Fz7 accumulations form on contacting plasma membranes Fz7-YFP and Fz7-CFP accumulation

8. Wnt11 induces the accumulation of Fz7 together with the Dsh at the plasma membrane in regions of cell contact. Dsh is recruited to Wnt11-induced Fz7 accumulations

9. Wnt11-induced Fz7 accumulations increase the persistence of cell contacts. Wnt11-induced accumulations of Fz7 modulate cell contact persistence

10. Wnt11-induced accumulations of Fz7 modulate cell contact persistence

11. What is Flamingo? Wnt11 and Fmi control convergent extension movements during zebrafish gastrulation. Formstone et al. Dev Biol. (2005) Usui et al. Cell. (1999) Veeman et al. Dev Cell. (2003) Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled. Seifert et al. Nat Rev Genet. (2007)

12. Fmi2 is able to induce accumulation of itself and Fz7 independently of Wnt11 Fmi2 colocalizes with Fz7 and Wnt11 at cell contacts and increases persistence

13. Fmi2 colocalizes with Fz7 and Wnt11 at cell contacts and increases persistence Fmi MO : Fmi morppholinos targeted against fmi1a, fmi1b, fmi2

14. Endogenous subcellular sites of Wnt11 and Fz7 activity marked by Dsh-YFP recruitment to the plasma membrane Slb mutant : inactive C-terminal truncated wnt11

15. Endogenous subcellular sites of Wnt11 and Fz7 activity marked by Dsh-YFP recruitment to the plasma membrane Fm464 : plasma membrane marker

16. Wnt11 persist at cell contacts and is required for contact persistence Wnt11-YFP + GPI-anchored RFP

17. Wnt11 persist at cell contacts and is required for contact persistence

18. Wnt11 Fz7 accumulation Dsh Dsh Dsh Fmi colocalizes with Fz7 Dsh Dsh accumulation PCP pathway Enhance cell adhesion Summary