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Optical microscopy

MENA3100, 14.01.09 OBK. Optical microscopy. Magnification Resolution Contrast. We want to see more details. Simple microscope. The simple microscope. Antony van Leeuwenhoek. (1632−1723). The compound microscope. ca. 1595 Hans Janssen. Robert Hook (1635−1703). Micrographia , 1664.

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Optical microscopy

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  1. MENA3100, 14.01.09 OBK Optical microscopy Magnification Resolution Contrast We want to see more details Simple microscope

  2. The simple microscope Antony van Leeuwenhoek (1632−1723)

  3. The compound microscope ca. 1595 Hans Janssen Robert Hook (1635−1703) Micrographia, 1664

  4. Stereo microscope

  5. Compound microscope for transmitted light

  6. Inverted compound microscope for reflected light

  7. Refraction Kromatisk abberation

  8. Spherical abberation

  9. The objective Corrections

  10. Numerical aperture (NA) NA = nsina Resolution R = 0,61l/NA Rayleigh criterion (Raleigh in Brandon and Kaplan)

  11. The limiting factor: Diffraction NA = nsina Oil immersion

  12. More is needed than just the objective Condenser system Eyepice Ocular (okular)

  13. Optical anisotropy Calcite

  14. Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices

  15. Optically active crystals in the microscope

  16. Optically active crystals in the microscope

  17. Michel-Levy chart

  18. Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: http://www.olympusmicro.com/primer/ http://www.microscopyu.com/ http://www.micro.magnet.fsu.edu/ http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf maintenance: http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/46-0009_e.pdf

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