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Bacterial Cell Walls

Bacterial Cell Walls. Cell membrane. Gram Stain. The most widely used staining procedure in bacteriology. Differential stain: differentiates between gram-positive and gram-negative bacteria. Bacteria that stain- purple are gram-positive pink are gram-negative.

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Bacterial Cell Walls

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  1. Bacterial Cell Walls

  2. Cell membrane

  3. Gram Stain • The most widely used staining procedure in bacteriology. • Differential stain: differentiates between gram-positive and gram-negative bacteria. • Bacteria that stain- • purpleare gram-positive • pink are gram-negative

  4. Gram Stain Technique: 1. The bacteria are first stained with crystal violet. 2. The bacteria are then treated with gram's iodine solution. This allows the stain to be retained better by forming an insoluble crystal violet-iodine complex. 3. A decolorizer, ethanol or acetone, is then added. This is the differential step. 4. The counterstainsafranin is applied. Since the gram-positive bacteria are already purple, they are not affected. Gram-negative bacteria become stained by the safranin.

  5. http://faculty.ccbcmd.edu/courses/bio141/labmanua/lab6/images/gram_stain_11.swfhttp://faculty.ccbcmd.edu/courses/bio141/labmanua/lab6/images/gram_stain_11.swf http://youtu.be/WrNckI6E3GA http://youtu.be/YpDglhibW4k

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