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Tet-inducible 3T3-L1 cells

LTR. GFP. CMV/TRE. PGK. Blast. LTR. Tet-inducible 3T3-L1 cells. RHKD lentivirus infection at <10%. Day 1. Seeding at 1000~2000 cells per well in 96-well plate, and induction with Dox. Day 2. Day 4. Induction of differentiation. Clone screening (total ~ 450,000). Day 10-12.

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Tet-inducible 3T3-L1 cells

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  1. LTR GFP CMV/TRE PGK Blast. LTR Tet-inducible 3T3-L1 cells RHKD lentivirus infection at <10% Day 1 Seeding at 1000~2000 cells per well in 96-well plate, and induction with Dox Day 2 Day 4 Induction of differentiation Clone screening (total ~ 450,000) Day 10-12 Clone pickup and grow up with blasticidin, but without Dox Re-differentiation with and without Dox Gene identification shRNA verification

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