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SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT

SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT. G . D oran , N. DeLappe, C. O Hare, G. Corbett – Feeney, M. Cormican National Salmonella Reference Laboratory, Galway. INTRODUCTION. S. TYPHIMURIUM COMMON IN EUROPE, USA POULTRY, CATTLE & PIGS MANY FOODS

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SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT

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  1. SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT G. Doran , N. DeLappe, C. O Hare, G. Corbett – Feeney, M. Cormican National Salmonella Reference Laboratory,Galway

  2. INTRODUCTION • S. TYPHIMURIUM COMMON IN EUROPE, USA • POULTRY, CATTLE & PIGS MANY FOODS • MULTIDRUG RESISTANT ISOLATES

  3. INTRODUCTION -2 • MULTI DRUG RESISTANT DT104 • INCIDENCE INCREASING • ACSSuT – CHROMOSOMALLY CARRIED • TRIMETHOPRIM, NALIDIXIC ACID RESISTANCE • SUSCEPTIBILITY TO CIPROFLOXACIN

  4. TYPING METHODS • PHAGE TYPING • ANTIMICROBIAL SUSCEPTIBILITY • PLASMID PROFILING • PFGE

  5. PRINCIPLES OF PFGE CATTTTAC GTAAAATG

  6. PRINCIPLES OF PFGE CATGTTAC GTACAATG NO CUT

  7. PULSENET • STANDARDISED PFGE • CDC USA • E. coli, Salmonella, Listeria, Shigella • CENTRAL DATABASE

  8. PFGE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

  9. PLASMID PROFILE Size (MDa) 1 2 3 4 5 C C C C  6 7 8 9 10 125 26  10  2 

  10. S. TYPHIMURIUM 2001 • 303 ISOLATES • ANIMAL AND HUMAN ORIGIN • PHAGE TYPING (N = 303) • ANTIMICROBIAL SUSCEPTIBILITY (N = 303) • PGFE TYPING (N = 163) • PLASMID PROFILING (N = 36)

  11. ANTIMICROBIAL SUSCEPTIBILITY

  12. PLASMID PROFILING • 36 ISOLATES • MODIFIED METHOD OF KADO AND LIU • PLASMIDS 1.5 - 125 MDA • 60MDA PLASMID IN DT104, MDR DT120 AND DT12

  13. PATTERN PHAGE TYPE MDR A DT104, DT104b, MDR DT12 &120 88% MDR B C 32 ISOLATES FULLY SENS D E F DT12 DT120 G H

  14. PHAGE TYPE U310 • EMERGED IN 2001 • PIGS, HUMANS • 15 ISOLATES • MINOR DIFFERENCES ON PFGE

  15. DISCUSSION • S. TYPHIMURIUM – • HUMANS & ANIMALS • MDR - INCREASED • DT104 & DT104b • MAJORITY MDR, CLONAL

  16. DISCUSSION - 2 DT104, DT104b PFGEU302, RDNC P11 MDR DT12, DT120 P3, P5, P7, P14 DT193 P18, P20 P23, P27

  17. CONCLUSIONS • PHENOTYPIC & GENOTYPIC METHODS NECESSARY • HIGH INCIDENCE OF MDR IN IRELAND • CONTINUOUS MONITORING OF S. TYPHIMURIUM NECESSARY

  18. OTHER RESEARCH • FLUROQUINOLONE RESISTANCE IN S. ENTERITIDIS • DONNA KILMARTIN

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