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Dynamic Electroporation of Water

Dynamic Electroporation of Water. Jacob Frye, Courtney Green, Christopher Robertson, and Scott Shields. Introduction. Water is the most important resource. chemicals(chlorine, iodine) are widely used for sterilization. Development of alternative sterilization using electricity. Background.

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Dynamic Electroporation of Water

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  1. Dynamic Electroporation of Water Jacob Frye, Courtney Green, Christopher Robertson, and Scott Shields

  2. Introduction • Water is the most important resource. • chemicals(chlorine, iodine) are widely used for sterilization. • Development of alternative sterilization using electricity.

  3. Background • Electroporation – forcefully opening pores of bacteria. • Electrical team performed static electroporation tests. • Results more inclined towards repetition and low voltage.

  4. Background cont. • Use of E. coli k12 to minimize risk while properly modeling bacteria response to electroporation. • Group certification to handle high voltage sources.

  5. Escherichia coli K12

  6. Escherichia coli K12 • Safe and Harmless. • Similar Properties to Common or Lethal E. Coli • Optima Growth at 37 degrees Celsius • Kill rate exists at 42 degrees Celsius

  7. Biosafety Qualifications • To work in the biological lab, Scott and Jacob had to pass the biosafety lab examination and read the Biosafety lab manual • Strict guidelines for the type of clothing and to properly test E. coli K12 around other dangerous bacteria.

  8. Grow Process of E. coli K12 • Plate preparation • Growing process • Incubation period • Treatment and testing • Growing treated bacteria • Determine bacterial reduction

  9. Specific Phases of E. coli K12 • Lag Phase • Log/Exponential Phase • Stationary Phase • Death Phase

  10. Design Parameters • Use results from previous electrical team to develop mechanical flow system. • 100% kill rate of bacteria • 5 kV/cm³ to kill bacteria • Attain an 8 oz. cup of drinkable water in under 10 minutes

  11. Figure 1: Primary Design of Fluid Chamber

  12. Figure 2: Secondary Design (Implementation Pending Time Constraints)

  13. Figure 3: Peristaltic Pump used to generate flow

  14. Manufacturing • Prefabricated Acrylic Rectangle Tubing • Lathe turned connectors • 16 gauge silver sheet • 8 gauge silver rod lathe turned ends • Interference press fit connection • Silicon adhesive for water containment

  15. Figure 4: Fluid Chamber with silver plates connected to Peristaltic Pump

  16. Preliminary Testing • Determine the possible flow rates with the Peristaltic Pump. • Tested the chamber at maximum flow rate. • Tested the chamber at our design flow rate. • Video and Still Images to follow.

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