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Electroporation the phenomenon and technical   approach to experiments

Electroporation the phenomenon and technical   approach to experiments. Peter Kramar University of Ljubljana Faculty of Electrical Engineering http://lbk.fe.uni-lj.si peter.kramar@fe.uni-lj.si. Wroclaw, 24.4.2009. Laboratory of Biocybernetics. http://lbk.fe.uni-lj.si. The phenomena.

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Electroporation the phenomenon and technical   approach to experiments

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  1. Electroporationthe phenomenon and technical  approach to experiments Peter Kramar University of Ljubljana Faculty of Electrical Engineering http://lbk.fe.uni-lj.si peter.kramar@fe.uni-lj.si Wroclaw, 24.4.2009

  2. Laboratory of Biocybernetics http://lbk.fe.uni-lj.si

  3. The phenomena

  4. The phenomena

  5. Applications Reversibleelectroporation: Cell fusion Gene transfection Drug delivery Cancer treatment Ireversible electroporation: Food pasterization Tissueablation

  6. Key issue The phenomena is already used (even in clinical applications) and it works well in several applications, but the basic principal are not clarified yet.

  7. Levels of electroporation • Planar lipid bilayers • Lipid vesicles • Cells in suspension • Attached cells • Cell organelles • Tissue

  8. Levels of electroporation • Planar lipid bilayers • Lipid vesicles • Cells in suspension • Attached cells • Cell organelles • Tissue

  9. Previous experiments

  10. Experimental systems Voltage clamp Kramar et al, Bioelectrochemistry 2007 Current clamp Kalinowski et al, Meas Sci Technol1995

  11. Measurement protocol

  12. Membrane formation 1-palmitoyl 2-oleoyl phosphatidylcholine POPC 1-palmitoyl 2-oleoyl phosphatidylserine POPS POPC:POPS mixtures 1:3, 1:1and 3:1 Lipids Aqueous solution Rising the level of aqueous solution Montal M., Mueller P. PNAS69: 3561-3566, 1972

  13. Capacitance measurement Discharge method Benz et al, ???? Capacitance to period converterKalinowski et al, 1995

  14. Voltage clamp - linear rising signal kU Kramar et al., 2007

  15. Current clamp - linear rising signal

  16. http://www.cliniporator.com/ect

  17. Thank you for your attention!

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