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Lab 27 Goals and Objectives: **NO DISINFECTING, NO HAND WASHING before 33&36!!!**

Lab 27 Goals and Objectives: **NO DISINFECTING, NO HAND WASHING before 33&36!!!** Exercise 31: Lethal Effect of UV Light Score plates (+++, ++, +, -) for growth using control side of each plate as +++ for comparison; record on class sheet

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Lab 27 Goals and Objectives: **NO DISINFECTING, NO HAND WASHING before 33&36!!!**

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  1. Lab 27 Goals and Objectives: **NO DISINFECTING, NO HAND WASHING before 33&36!!!** Exercise 31: Lethal Effect of UV Light Score plates (+++, ++, +, -) for growth using control side of each plate as +++ for comparison; record on class sheet Exercise 33: Effectiveness of Alcohol as an Antiseptic Each person do, follow supplemental packet directions p.113 Exercise 36: Effectiveness of Hand Scrubbing Four groups total: each group elect a scrubber and scrubber assistant, Others do plating; Use solid pre-poured plates, plate using flame sterilized spreader, 10 plates per group: 1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml) 3 basin B (0.1ml, 0.2ml, 0.4ml), 3 basin C (0.1ml, 0.2ml, 0.4ml) Exercise 34: Antimicrobial Sensitivity Testing Four groups: each test all 8 antibiotics against two organisms (assigned), two plates per organism, 4 discs per plate. Exercise 35: Evaluation of Antiseptics Each try five agents against both organisms S. aureus & P. aeruginosa SAME five agents for both to compare Gram pos. vs. neg.

  2. Replacement Directions for Exercise 33 Effectiveness of Alcohol as an Antiseptic Supp. page 125 A B Divide plate into 6 wedges F C E D A = left/non-dominant thumb dirty B = left/non-dominant thumb again (how many microbes removed just by touching agar?) C = right/dominant thumb dirty D = right/dominant thumb dipped in alcohol and allowed to dry (how many microbes removed/killed by alcohol dip? E = right/dominant index finger F = right/dominant index finger wiped with alcohol swab (how many microbes removed/killed by swabbing with alcohol?)

  3. Fig. 36.1 Stop at step 5 while you still have skin!

  4. 10 plates total: 1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml) 3 basin B (0.1ml, 0.2ml, 0.4ml) 3 basin C (0.1ml, 0.2ml, 0.4ml)

  5. Exercise 34: 2 plates each organism, 4 discs per plate Each organism gets tested against 8 different antibiotics Group 1 Staphylococcus aureus & Escherichia coli Group 2 Staphylococcus aureus & Proteus vulgaris Group 3 Proteus vulgaris & Pseudomonas aeruginosa Group 4 Escherichia coli & Pseudomonas aeruginosa

  6. Lab 27 Goals and Objectives: **NO DISINFECTING, NO HAND WASHING before 33&36!!!** Exercise 31: Lethal Effect of UV Light Score plates (+++, ++, +, -) for growth using control side of each plate as +++ for comparison; record on class sheet Exercise 33: Effectiveness of Alcohol as an Antiseptic Each person do, follow supplemental packet directions p.113 Exercise 36: Effectiveness of Hand Scrubbing Four groups total: each group elect a scrubber and scrubber assistant, Others do plating; Use solid pre-poured plates, plate using flame sterilized spreader, 10 plates per group: 1 tap water control (0.4ml), 3 basin A (0.1ml, 0.2ml, 0.4ml) 3 basin B (0.1ml, 0.2ml, 0.4ml), 3 basin C (0.1ml, 0.2ml, 0.4ml) Exercise 34: Antimicrobial Sensitivity Testing Four groups: each test all 8 antibiotics against two organisms (assigned), two plates per organism, 4 discs per plate. Exercise 35: Evaluation of Antiseptics Each try five agents against both organisms S. aureus & P. aeruginosa SAME five agents for both to compare Gram pos. vs. neg.

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