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Review of Synthetic Oscillatory Network: The Repressilator Study

Explore the synthesis of a functional oscillatory network in transcriptional regulators. Discover the limitations, controls, and iGEM solution along with deterministic and stochastic models.

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Review of Synthetic Oscillatory Network: The Repressilator Study

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  1. Synthetic Biology Review A Synthetic Oscillatory Network of Transcriptional Regulators – ‘The Repressilator’ Elowitz, M.B. & Leibler, S. Nature, vol. 403, 20th January 2000

  2. The Basics

  3. The Good Results...

  4. ...and the Other Results • ◄ Limitations (c): • Accumulation of GFP as a result of limited elimination • Green – oscillation with a reduced period • Blue – several periods skipped entirely • ◄ Controls (f): • Cells containing only the reporter plasmid Stochastic effects…

  5. Conclusions & Extensions • ◄ iGEM Solution: • Novel approach based on Lotka-Volterra model of predator-prey interaction • Constitutively repressed ‘predator’ cell is anti-repressed by product of ‘prey’ cell • Prey cell is repressed by product of predator cell… • Thus a stable oscillation cycle is built up Cell A A Lux I Plux Lux R A Cell B A A LuxR AiiA LuxR aiiA Plux Lux R

  6. The Network Model Deterministic Continuous / Stochastic Discrete Continuous model:

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