HIF-2 

1. Figure S1 parp-1 +/+ parp-1 -/- H N H N HIF-2 DAPI Fig S1. Nuclear HIF-2 accumulation by hypoxia is reduced in parp-1 -/- immortalizedMEFs.Immunofluorescence staining of parp-1 +/+ and parp-1 -/- immortalized MEFs incubated in normoxia (N) or hypoxia (H, 16 hours at 1 % O2). Representative images from several independent experiments are shown.

2. Figure S2 H H PARP-1 HIF-1α Control HIF-2α Control Control siRNA: siRNA: siRNA: HIF-1α HIF-2α PARP-1 β-Actin β-Actin β-Actin Figure S2. Validation of silencing efficacy: siRNA of PARP-1, HIF-2 or HIF-1. (Cells were transfected with the correspondig siRNAs and 48h later, the expression levels of the targeted protein was evaluated by WB. HIF expression was analyzed after 16 hours of exposure to hypoxia (1% O2).

3. Figure S3 parp-1+/+ 75 15 15 Liver Kidney Brain parp-1-/- 50 10 10 Epo mRNA expression (fold induction) N N N H H H * 25 5 5 ** 0 0 0 Figure S3. EPO mRNA levels were determined by Q-PCR from liver, kidney and brain . The level of mRNA in each sample was expressed as fold of the value in control wild-type mice. Numeric value represents mean of hypoxic induction in each genotype at 8 hours, respectively. (*) and (**) indicate p< 0.05 and p<0.001, with respect to wild-type mice.